Melbourne/Lab Notebook Weeks 13-16

From 2007.igem.org

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*[[Melbourne/Miniprep protocol|Miniprepped]] 2 cultures from each of the transformations on 12/9 and 4 cultures from Omp-C1-reporter.
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*[[Melbourne/Diagnostic Digest| Digested]] these and [[Melbourne/Loading a DNA gel|Ran on a Gel]]:
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Gel1:
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*# [[Melbourne/primary DNA marker|DNA ladder]]
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*# P17OA E/H
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*# P17OB E/H
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*# [[Melbourne/BBa_C0051|P1 5G]] A E/H
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*# [[Melbourne/BBa_C0051|P1 5G]] B E/H
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*# Omp-C1-reporter A X/P
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*# Omp-C1-reporter B X/P
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*# Omp-C1-reporter C X/P
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*# Omp-C1-reporter D X/P
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*# [[Melbourne/BBa_I15008|P2 21A]] A X/P
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*# [[Melbourne/BBa_I15008|P2 21A]] B X/P
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*# [[Melbourne/BBa_I15009|P2 21C]] A X/P
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*# [[Melbourne/BBa_I15009|P2 21C]] B X/P
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*# Death-ComA 5min ligation A X/P
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*# Death-ComA 5min ligation B X/P
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*# Death-ComA overnight ligation A X/P
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*# Death-ComA overnight ligation B X/P
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*# [[Melbourne/primary DNA marker|DNA ladder]]
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Gel2:
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*# [[Melbourne/primary DNA marker|DNA ladder]]
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*# Death-ComP A X/P
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*# Death-ComP B X/P
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*# Cph8 A X/P
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*# Cph8 B X/P
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==Week 14==
==Week 14==

Revision as of 12:26, 9 October 2007

<Return to Lab notebook> <team home page>

Contents

Week 13

16 Sept 2007


17 Sept 2007

Gel1:

    1. DNA ladder
    2. P17OA E/H
    3. P17OB E/H
    4. P1 5G A E/H
    5. P1 5G B E/H
    6. Omp-C1-reporter A X/P
    7. Omp-C1-reporter B X/P
    8. Omp-C1-reporter C X/P
    9. Omp-C1-reporter D X/P
    10. P2 21A A X/P
    11. P2 21A B X/P
    12. P2 21C A X/P
    13. P2 21C B X/P
    14. Death-ComA 5min ligation A X/P
    15. Death-ComA 5min ligation B X/P
    16. Death-ComA overnight ligation A X/P
    17. Death-ComA overnight ligation B X/P
    18. DNA ladder

Gel2:

    1. DNA ladder
    2. Death-ComP A X/P
    3. Death-ComP B X/P
    4. Cph8 A X/P
    5. Cph8 B X/P


18 Sept 2007


19 Sept 2007


20 Sept 2007


21 Sept 2007


22 Sept 2007

Week 14

23 Sept 2007


24 Sept 2007


25 Sept 2007


26 Sept 2007

  • Miniprepped the following
    • TetR-RBS-P2,21A-ter A,B,C and D
    • TetR-RBS-ComP-ter A,B,C and D
    • GenRBS-ComA-ter A,B,C and D
    • cI-L3 A,B,C and D

27 Sept 2007


28 Sept 2007


29 Sept 2007


Week 15

30 Sept 2007


1 Oct 2007


2 Oct 2007


3 Oct 2007


4 Oct 2007


5 Oct 2007


6 Oct 2007


Week 16

7 Oct 2007


8 Oct 2007


9 Oct 2007


10 Oct 2007


11 Oct 2007


12 Oct 2007


13 Oct 2007

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