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Revision as of 08:20, 5 October 2007 (view source) Blent (Talk | contribs) (→PCR length check) ← Older edit		Revision as of 08:21, 5 October 2007 (view source) Blent (Talk | contribs) (→PCR length check) Newer edit →
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	== PCR length check ==		== PCR length check ==
		+	* PCR product length without any insert = (3,189 - 3,049) + 176 = 316 by primers VF2 and VR
	* expected lengths of three ligations		* expected lengths of three ligations
	** (I) pCinRHSL (vector) + OmpRBS (insert):		** (I) pCinRHSL (vector) + OmpRBS (insert):
	** (II) pOmpC (vector) + TATA_INSA (insert)		** (II) pOmpC (vector) + TATA_INSA (insert)
	** (III) TATB_INSB (insert) + CinR+HSL+D-term (vector)		** (III) TATB_INSB (insert) + CinR+HSL+D-term (vector)

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Revision as of 08:21, 5 October 2007

PCR length check

• PCR product length without any insert = (3,189 - 3,049) + 176 = 316 by primers VF2 and VR
• expected lengths of three ligations
  • (I) pCinRHSL (vector) + OmpRBS (insert):
  • (II) pOmpC (vector) + TATA_INSA (insert)
  • (III) TATB_INSB (insert) + CinR+HSL+D-term (vector)