NYMU Taipei/Lab Notes/2007 10 5

From 2007.igem.org

(Difference between revisions)
(→PCR length check)
(→PCR length check)
Line 3: Line 3:
* expected lengths of three ligations
* expected lengths of three ligations
** (I) pCinRHSL (vector) + OmpRBS (insert): 291 + 316 = 613
** (I) pCinRHSL (vector) + OmpRBS (insert): 291 + 316 = 613
 +
*** 291 = pCinRHSL (225) + Sequence after left cutting sites (1) + OmpRBS (65)
** (II) pOmpC (vector) + TATA_INSA (insert): 297 + 316 = 603
** (II) pOmpC (vector) + TATA_INSA (insert): 297 + 316 = 603
 +
*** 297 = pOmpC (108) + Sequence after left cutting sites (23) + TATA (90) + INSA (66)
** (III) TATB_INSB (insert) + CinR+HSL+D-term (vector): 1,829 + 316 = 2,145
** (III) TATB_INSB (insert) + CinR+HSL+D-term (vector): 1,829 + 316 = 2,145

Revision as of 05:16, 9 October 2007

PCR length check

  • PCR product length without any insert = (3,189 - 3,049) + 176 = 316 by primers VF2 and VR
  • expected lengths of three ligations
    • (I) pCinRHSL (vector) + OmpRBS (insert): 291 + 316 = 613
      • 291 = pCinRHSL (225) + Sequence after left cutting sites (1) + OmpRBS (65)
    • (II) pOmpC (vector) + TATA_INSA (insert): 297 + 316 = 603
      • 297 = pOmpC (108) + Sequence after left cutting sites (23) + TATA (90) + INSA (66)
    • (III) TATB_INSB (insert) + CinR+HSL+D-term (vector): 1,829 + 316 = 2,145
Retrieved from "http://2007.igem.org/wiki/index.php/NYMU_Taipei/Lab_Notes/2007_10_5"