Melbourne/Lab BL Notebook/PrimersI
From 2007.igem.org
(Difference between revisions)
| Line 7: | Line 7: | ||
Sequence: Cagtcggtgcggacatcgttgcttgtaagcaaagcttatac | Sequence: Cagtcggtgcggacatcgttgcttgtaagcaaagcttatac | ||
Number of Bases: 41 | Number of Bases: 41 | ||
| + | Tm:68 | ||
-- Oligo 2 -- | -- Oligo 2 -- | ||
Sequence Name: BL_Con1_as | Sequence Name: BL_Con1_as | ||
Sequence: GTATAAGCTTTGCTTACAAGCAACGATGTCCGCACCGACTG | Sequence: GTATAAGCTTTGCTTACAAGCAACGATGTCCGCACCGACTG | ||
Number of Bases: 41 | Number of Bases: 41 | ||
| + | Tm:68 | ||
-- Oligo 3 -- | -- Oligo 3 -- | ||
Sequence Name: BL_Con2_s | Sequence Name: BL_Con2_s | ||
Sequence: TcggtgcggacatcgctgCTTGTAAGCAAAGCTTATACCTTTG | Sequence: TcggtgcggacatcgctgCTTGTAAGCAAAGCTTATACCTTTG | ||
Number of Bases: 43 | Number of Bases: 43 | ||
| + | Tm:69 | ||
-- Oligo 4 -- | -- Oligo 4 -- | ||
Sequence Name: BL_Con2_as | Sequence Name: BL_Con2_as | ||
Sequence: CAAAGGTATAAGCTTTGCTTACAAGCAGCGATGTCCGCACCGA | Sequence: CAAAGGTATAAGCTTTGCTTACAAGCAGCGATGTCCGCACCGA | ||
Number of Bases: 43 | Number of Bases: 43 | ||
| + | Tm:69 | ||
-- Oligo 5 -- | -- Oligo 5 -- | ||
Sequence Name: BL_Con3_s | Sequence Name: BL_Con3_s | ||
Sequence: GGTGCGGACATCGCTGGAGGTAAGCAAAGCTTATACCTTTGAG | Sequence: GGTGCGGACATCGCTGGAGGTAAGCAAAGCTTATACCTTTGAG | ||
Number of Bases: 43 | Number of Bases: 43 | ||
| + | Tm:70 | ||
-- Oligo 6 -- | -- Oligo 6 -- | ||
Sequence Name: BL_Con3_as | Sequence Name: BL_Con3_as | ||
Sequence: CTCAAAGGTATAAGCTTTGCTTACCTCCAGCGATGTCCGCACC | Sequence: CTCAAAGGTATAAGCTTTGCTTACCTCCAGCGATGTCCGCACC | ||
Number of Bases: 43 | Number of Bases: 43 | ||
| + | Tm:70 | ||
-- Oligo 7 -- | -- Oligo 7 -- | ||
Sequence Name: BL_Con4_s | Sequence Name: BL_Con4_s | ||
Sequence: GCGGACATCGCTGGAGGACAGCAAAGCTTATACCTTTGAGGT | Sequence: GCGGACATCGCTGGAGGACAGCAAAGCTTATACCTTTGAGGT | ||
Number of Bases: 42 | Number of Bases: 42 | ||
| + | Tm:70 | ||
-- Oligo 8 -- | -- Oligo 8 -- | ||
Sequence Name: BL_Con4_as | Sequence Name: BL_Con4_as | ||
Sequence: ACCTCAAAGGTATAAGCTTTGCTGTCCTCCAGCGATGTCCGC | Sequence: ACCTCAAAGGTATAAGCTTTGCTGTCCTCCAGCGATGTCCGC | ||
Number of Bases: 42 | Number of Bases: 42 | ||
| + | Tm:70 | ||
-- Oligo 9 -- | -- Oligo 9 -- | ||
Sequence Name: BL_Con5_s | Sequence Name: BL_Con5_s | ||
Sequence: GACATCGCTGGAGGACGCCAAAGCTTATACCTTTGAGGTTACTC | Sequence: GACATCGCTGGAGGACGCCAAAGCTTATACCTTTGAGGTTACTC | ||
Number of Bases: 44 | Number of Bases: 44 | ||
| + | Tm:70 | ||
-- Oligo 10 -- | -- Oligo 10 -- | ||
Sequence Name: BL_Con5_as | Sequence Name: BL_Con5_as | ||
Sequence: GAGTAACCTCAAAGGTATAAGCTTTGGCGTCCTCCAGCGATGTC | Sequence: GAGTAACCTCAAAGGTATAAGCTTTGGCGTCCTCCAGCGATGTC | ||
Number of Bases: 44 | Number of Bases: 44 | ||
| + | Tm:70 | ||
-- Oligo 11 -- | -- Oligo 11 -- | ||
Sequence Name: BL_Con6_s | Sequence Name: BL_Con6_s | ||
Sequence: gacgcagagcaggcacaaaagGTGATATTTTTAGATAAGCATGAAGTT | Sequence: gacgcagagcaggcacaaaagGTGATATTTTTAGATAAGCATGAAGTT | ||
Number of Bases: 48 | Number of Bases: 48 | ||
| + | Tm:67 | ||
-- Oligo 12 -- | -- Oligo 12 -- | ||
Sequence Name: BL_Con6_as | Sequence Name: BL_Con6_as | ||
Sequence: AACTTCATGCTTATCTAAAAATATCACCTTTTGTGCCTGCTCTGCGTC | Sequence: AACTTCATGCTTATCTAAAAATATCACCTTTTGTGCCTGCTCTGCGTC | ||
Number of Bases: 48 | Number of Bases: 48 | ||
| + | Tm:67 | ||
-- Oligo 13 -- | -- Oligo 13 -- | ||
Sequence Name: BL_Con7_s | Sequence Name: BL_Con7_s | ||
Sequence: GGCAGAGGAGATCAACACGGAAGTTGGACCGGACTGGAATT | Sequence: GGCAGAGGAGATCAACACGGAAGTTGGACCGGACTGGAATT | ||
Number of Bases: 41 | Number of Bases: 41 | ||
| + | Tm:70 | ||
-- Oligo 14 -- | -- Oligo 14 -- | ||
Sequence Name: BL_Con7_as | Sequence Name: BL_Con7_as | ||
Sequence: AATTCCAGTCCGGTCCAACTTCCGTGTTGATCTCCTCTGCC | Sequence: AATTCCAGTCCGGTCCAACTTCCGTGTTGATCTCCTCTGCC | ||
Number of Bases: 41 | Number of Bases: 41 | ||
| + | Tm:70 | ||
-- Oligo 15 -- | -- Oligo 15 -- | ||
Sequence Name: BL_FP1_s | Sequence Name: BL_FP1_s | ||
Sequence: AGCTAGGaattcgcggccgcttctagATGGTGGGACTTACGACCCTCT | Sequence: AGCTAGGaattcgcggccgcttctagATGGTGGGACTTACGACCCTCT | ||
Number of Bases: 48 | Number of Bases: 48 | ||
| - | + | Tm:74 | |
| - | + | ||
| - | + | ||
| - | + | ||
Revision as of 12:20, 10 October 2007
Primers used for PCR stitching of Blue photoreceptor
Copied and pasted from order confirmation email (sans price)
-- Oligo 1 --
Sequence Name: BL_Con1_s Sequence: Cagtcggtgcggacatcgttgcttgtaagcaaagcttatac Number of Bases: 41 Tm:68
-- Oligo 2 --
Sequence Name: BL_Con1_as Sequence: GTATAAGCTTTGCTTACAAGCAACGATGTCCGCACCGACTG Number of Bases: 41 Tm:68
-- Oligo 3 --
Sequence Name: BL_Con2_s Sequence: TcggtgcggacatcgctgCTTGTAAGCAAAGCTTATACCTTTG Number of Bases: 43 Tm:69
-- Oligo 4 --
Sequence Name: BL_Con2_as Sequence: CAAAGGTATAAGCTTTGCTTACAAGCAGCGATGTCCGCACCGA Number of Bases: 43 Tm:69
-- Oligo 5 --
Sequence Name: BL_Con3_s Sequence: GGTGCGGACATCGCTGGAGGTAAGCAAAGCTTATACCTTTGAG Number of Bases: 43 Tm:70
-- Oligo 6 --
Sequence Name: BL_Con3_as Sequence: CTCAAAGGTATAAGCTTTGCTTACCTCCAGCGATGTCCGCACC Number of Bases: 43 Tm:70
-- Oligo 7 --
Sequence Name: BL_Con4_s Sequence: GCGGACATCGCTGGAGGACAGCAAAGCTTATACCTTTGAGGT Number of Bases: 42 Tm:70
-- Oligo 8 --
Sequence Name: BL_Con4_as Sequence: ACCTCAAAGGTATAAGCTTTGCTGTCCTCCAGCGATGTCCGC Number of Bases: 42 Tm:70
-- Oligo 9 --
Sequence Name: BL_Con5_s Sequence: GACATCGCTGGAGGACGCCAAAGCTTATACCTTTGAGGTTACTC Number of Bases: 44 Tm:70
-- Oligo 10 --
Sequence Name: BL_Con5_as Sequence: GAGTAACCTCAAAGGTATAAGCTTTGGCGTCCTCCAGCGATGTC Number of Bases: 44 Tm:70
-- Oligo 11 --
Sequence Name: BL_Con6_s Sequence: gacgcagagcaggcacaaaagGTGATATTTTTAGATAAGCATGAAGTT Number of Bases: 48 Tm:67
-- Oligo 12 --
Sequence Name: BL_Con6_as Sequence: AACTTCATGCTTATCTAAAAATATCACCTTTTGTGCCTGCTCTGCGTC Number of Bases: 48 Tm:67
-- Oligo 13 --
Sequence Name: BL_Con7_s Sequence: GGCAGAGGAGATCAACACGGAAGTTGGACCGGACTGGAATT Number of Bases: 41 Tm:70
-- Oligo 14 --
Sequence Name: BL_Con7_as Sequence: AATTCCAGTCCGGTCCAACTTCCGTGTTGATCTCCTCTGCC Number of Bases: 41 Tm:70
-- Oligo 15 --
Sequence Name: BL_FP1_s Sequence: AGCTAGGaattcgcggccgcttctagATGGTGGGACTTACGACCCTCT Number of Bases: 48 Tm:74
