Samantha Liang Notebook

From 2007.igem.org

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[[Template:BerkiGEM2007_SamanthaBiobricks | My Biobricks]]<br>
[[Template:BerkiGEM2007_SamanthaBiobricks | My Biobricks]]<br>
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==--[[User:Samanthaliang|Samanthaliang]] 15:29, 4 June 2007 (EDT)==
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==[[User:Samanthaliang|Samanthaliang]] 15:29, 4 June 2007 (EDT)==
*Oligos to make biobricks of toxins are not yet in - maybe Wednesday or Thursday?
*Oligos to make biobricks of toxins are not yet in - maybe Wednesday or Thursday?
'''For cassette between Cre and toxin: [rbs][ATG][Lox][Term][Lox]''' <br>
'''For cassette between Cre and toxin: [rbs][ATG][Lox][Term][Lox]''' <br>

Revision as of 19:31, 4 June 2007

My Construction Files
My Sequencing Files
My Biobricks


Samanthaliang 15:29, 4 June 2007 (EDT)

  • Oligos to make biobricks of toxins are not yet in - maybe Wednesday or Thursday?

For cassette between Cre and toxin: [rbs][ATG][Lox][Term][Lox]

  • Made [rbs][ATG] variants with different ribosome binding sites
       I716201 is Bca1106A.Bca1112 
I716202 is Bca1106B.Bca1112
I716203 is Bca1128A.Bca1112
I716204 is Bca1090.Bca1112
  • Made [Lox][Term] variants with 2 different terminators (will probably use Bca1092 version)
     I716205 is Bca1114.Bca1124 
I716206 is Bca1114.Bca1092
  • Tomorrow will make [Lox][Term][Lox] part
  • Then will put the rbsATG together with LoxTermLox

eek so many construction files and ape files to make.


to do