BerkiGEM2007-SamanthaConstructionFile5
From 2007.igem.org
(Difference between revisions)
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PCR sil04F and sil04R on BamHI methylase/endonuclease region. (657 bp, BglII/XhoI) <br> | PCR sil04F and sil04R on BamHI methylase/endonuclease region. (657 bp, BglII/XhoI) <br> | ||
Sub into pBca9145-Bca1089 (BglII/XhoI,2063+696, L) <br> | Sub into pBca9145-Bca1089 (BglII/XhoI,2063+696, L) <br> | ||
| - | Product is pBca9145- | + | Product is pBca9145-I716210 <br> |
---------------------------- | ---------------------------- | ||
sil04F Forward BglII biobrick of BamHI <br> ccaaaAGATCTgaagtagaaaaagagtttattactga <br> | sil04F Forward BglII biobrick of BamHI <br> ccaaaAGATCTgaagtagaaaaagagtttattactga <br> | ||
sil04R Reverse XholI/BamHI biobrick of BamHI <br> gctagCTCGAGttaGGATCCctatttgttttcaactttatc <br> | sil04R Reverse XholI/BamHI biobrick of BamHI <br> gctagCTCGAGttaGGATCCctatttgttttcaactttatc <br> | ||
Latest revision as of 18:23, 5 June 2007
Biobricking of BamHI endonuclease without start codon
PCR sil04F and sil04R on BamHI methylase/endonuclease region. (657 bp, BglII/XhoI)
Sub into pBca9145-Bca1089 (BglII/XhoI,2063+696, L)
Product is pBca9145-I716210
sil04F Forward BglII biobrick of BamHI
ccaaaAGATCTgaagtagaaaaagagtttattactga
sil04R Reverse XholI/BamHI biobrick of BamHI
gctagCTCGAGttaGGATCCctatttgttttcaactttatc
