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	==[[User:Samanthaliang|Samanthaliang]] 19:20, 5 June 2007 (EDT)==		==[[User:Samanthaliang|Samanthaliang]] 19:20, 5 June 2007 (EDT)==

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Revision as of 23:20, 5 June 2007

My Construction Files
My Sequencing Files
My Biobricks

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Samanthaliang 19:20, 5 June 2007 (EDT)

• Grew up 2 colonies each of I716201 through I716206
• Made a lot of construction files and entered things into the registry
• My oligos were ordered today!
• Demonstrated liquid media making and registry entering
• Now will also make an RFP variant as a reporter on how well Cre is working

To do tomorrow:

• subclone 207 and 208
• Run PCRs if they come in the afternoon
• Send things out for sequencing and make -80s
• Fill our and sent those survey forms

Samanthaliang 15:29, 4 June 2007 (EDT)

• Oligos to make biobricks of toxins are not yet in - maybe Wednesday or Thursday?

For cassette between Cre and toxin: [rbs][ATG][Lox][Term][Lox]

• Made [rbs][ATG] variants with different ribosome binding sites

       I716201 is Bca1106A.Bca1112 

       I716202 is Bca1106B.Bca1112 

       I716203 is Bca1128A.Bca1112 

       I716204 is Bca1090.Bca1112 

• Made [Lox][Term] variants with 2 different terminators (will probably use Bca1092 version)

     I716205 is Bca1114.Bca1124 

      I716206 is Bca1114.Bca1092 

• Tomorrow will make [Lox][Term][Lox] part
• Then will put the rbsATG together with LoxTermLox

eek so many construction files and ape files to make.

to do