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	::'''10/15/07'''		::'''10/15/07'''
	*O/N ligations for:		*O/N ligations for:
-	::-Plac-CI-LacY-GFP;	+	::-I763019;
	::-pTet-LacI-T-Plambda-RFP.		::-pTet-LacI-T-Plambda-RFP.
-	*Run on electrophoresis gel and Plac-CI-LacY-GFP band extraction.	+	*Run on electrophoresis gel and I763019 band extraction.
	::'''10/16/07'''		::'''10/16/07'''
-	*Transformation of Plac-CI-LacY-GFP and pTet-LacI-T-Plambda-RFP.	+	*Transformation of I763019 and pTet-LacI-T-Plambda-RFP.
	*We inoculate:		*We inoculate:
-	::-Plac-CI;	+	::-I763005;
-	::-RBS-LacY;	+	::-I763015;
-	::-Plac-GFP.	+	::-I763004.
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	::'''10/17/07'''		::'''10/17/07'''
	*Miniprep of:		*Miniprep of:
-	::-Plac-CI;	+	::-I763005;
-	::-RBS-LacY;	+	::-I763015;
-	::-Plac-GFP.	+	::-I763004.
	*We inoculate:		*We inoculate:
	::-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp;		::-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp;

---

Revision as of 09:23, 22 October 2007

10/15/07

• O/N ligations for:

-I763019;

-pTet-LacI-T-Plambda-RFP.

• Run on electrophoresis gel and I763019 band extraction.

10/16/07

• Transformation of I763019 and pTet-LacI-T-Plambda-RFP.
• We inoculate:

-I763005;

-I763015;

-I763004.

10/17/07

• Miniprep of:

-I763005;

-I763015;

-I763004.

• We inoculate:

-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp;

-pTet-LacI-T-pLambda-rfp (high copy).

éDigestion of

10/18/07

10/19/07

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