From 2007.igem.org

Revision as of 00:21, 8 June 2007 (view source) Samanthaliang (Talk | contribs) ← Older edit		Revision as of 18:54, 20 June 2007 (view source) Samanthaliang (Talk | contribs) Newer edit →
Line 1:		Line 1:
-
	'''[rbs][Cre]''' <br>		'''[rbs][Cre]''' <br>
-	'''rbs variants Bca1090''' <br>	+	'''rbs library - want a weak rbs actually''' <br>
-	'''2 Different Cre variants (212 and 213)''' <br>	+	'''3 Different Cre variants (ATG Bca1117, GTG I716212 and TTG I716213)''' <br>
	----		----
		+
		+	'''ATG Cre variants''' <br>
		+
		+	Digest pBca1121-Bca9230  (EcoRI/BamHI, 2927+955bp, Small) <br>
		+	Digest pBca9145-Bca1117  (EcoRI/BglII, 3101+9bp, Large)<br>
		+	Plate on CamAmp
		+	Product is pBca9145-I716224. <br>
		+
		+	Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small) <br>
		+	Digest pBca9145-Bca1117 (EcoRI/BglII, 3101+9bp, Large) <br>
		+	Plate on KanAmp
		+	Product is pBca9145-I716225. <br>
		+
		+
		+
		+	'''GTG Cre variants''' <br>
		+
		+	Digest pBca1121-Bca9230  (EcoRI/BamHI, 2927+955bp, Small) <br>
		+	Digest pBca9145-I716212  (EcoRI/BglII, 3101+9bp, Large)<br>
		+	Plate on CamAmp
		+	Product is pBca9145-I716224. <br>
		+
		+	Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small) <br>
		+	Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large) <br>
		+	Plate on KanAmp
		+	Product is pBca9145-I716225. <br>
		+
-	Digest pBca9145-Bca1090 (BamHI/BglI/AlwNI, 942+596+553bp, Large) <br>	+	'''TTG Cre variants''' <br>
-	Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small) <br>	+
-	Product is pBca9145-I716217. <br>	+
		+	Digest pBca1121-Bca9230  (EcoRI/BamHI, 2927+955bp, Small) <br>
		+	Digest pBca9145-I716213  (EcoRI/BglII, 3101+9bp, Large)<br>
		+	Plate on CamAmp
		+	Product is pBca9145-I716224. <br>
-	Digest pBca9145-Bca1090  (BamHI/BglI, 942+596+553bp, Large) <br>	+	Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small) <br>
-	Digest pBca9145-I716213   (BglII/BglI, 2187+923bp, Small)<br>	+	Digest pBca9145-I716213 (EcoRI/BglII, 3101+9bp, Large) <br>
-	Product is pBca9145-I716221. <br>	+	Plate on KanAmp
		+	Product is pBca9145-I716225. <br>

---

Revision as of 18:54, 20 June 2007

[rbs][Cre]
rbs library - want a weak rbs actually
3 Different Cre variants (ATG Bca1117, GTG I716212 and TTG I716213)

---

ATG Cre variants

Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-Bca1117 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp Product is pBca9145-I716224.

Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small)
Digest pBca9145-Bca1117 (EcoRI/BglII, 3101+9bp, Large)
Plate on KanAmp Product is pBca9145-I716225.

GTG Cre variants

Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp Product is pBca9145-I716224.

Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small)
Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large)
Plate on KanAmp Product is pBca9145-I716225.

TTG Cre variants

Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-I716213 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp Product is pBca9145-I716224.

Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small)
Digest pBca9145-I716213 (EcoRI/BglII, 3101+9bp, Large)
Plate on KanAmp Product is pBca9145-I716225.