Paris/October 19
From 2007.igem.org
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== Digestion reactions == | == Digestion reactions == | ||
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| + | == Digestion reactions == | ||
Revision as of 04:59, 22 October 2007
Digestion reactions
| Digestion Products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Size | Description | |||
| D22bis | pSB1A2 BBv | MP6.1 et .2 | EcoRI | PstI | pSB1A2 vector digested with EcoRI and PstI enzymes | ||||
| D80 | PacI[ptet>>lox71-gfpT-lox66-mrfp]SmaI | P15 | PacI | SmaI | ptet>>lox71-gfpT-lox66-mrfp P15 PCR product digested by PacI-SmaI for cloning into pY-2C-IntC | ||||
| D81 | PacI[pY-2C-IntC]SmaI | pY-2C-IntC plasmid | PacI | SmaI | pY-2C-IntC digested for ptet>>lox71-gfpT-lox66-mrfp cloning | ||||
| D88 | pTet>>lox71-gfptripqrt FV | L34.1 et .2 | EcoRI | XbaI | pTet>>lox71-gfptripqrt pSB1A2 dig. for use as a FV | ||||
| D89 | T-lox66-rbsdapAcoli BV | L53.7 | SpeI | PstI | T-lox66-rbsdapAcoli pSB1A2 dig. for use as a BV | ||||
| D90 | T-lox66-rbsdapAsubtilis BV | L56 | SpeI | PstI | T-lox66-rbsdapAsubtilis pSB1A2 dig. for use as a BV | ||||
| D91 | ptet>>lox71-gfpT-lox66-mrfp pSB1A2 BV | L59.6, .7 | SpeI | PstI | ptet>>lox71-gfpT-lox66-mrfp pSB1A2 dig. for use as a BV | ||||
| D92 | rbsdapAcoli BI | L39.1 | XbaI | PstI | rbsdapAcoli extracted for use as a BI | ||||
| D93 | rbsdapAsubtilis BI | L64.1, .2 | XbaI | PstI | rbsdapAsubtilis extracted for use as a BI | ||||
