Tokyo/sunaba2
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<br>[[Image:Bistability.JPG|200px]][[Image:Coexistence.JPG|250px]] | <br>[[Image:Bistability.JPG|200px]][[Image:Coexistence.JPG|250px]] | ||
| - | ===[[Tokyo/Genetic circuit|Genetic circuit]] | + | ===[[Tokyo/Genetic circuit|Genetic circuit]]=== |
| + | ====We constructed the genetic circuit to fill its requirements. Therefore we made [[Tokyo/new parts |new parts]] ==== | ||
<br>[[Image:Tokyo_genetic circuit_s.jpg|350px]] | <br>[[Image:Tokyo_genetic circuit_s.jpg|350px]] | ||
====Our construction parts are [[Tokyo/construction|Here]]!!! ==== | ====Our construction parts are [[Tokyo/construction|Here]]!!! ==== | ||
Revision as of 16:47, 21 October 2007
砂場2
1,ProjectConcept & ModelWe made bacterial society that follows Pareto's principle,for example ,Ant society
RequirementsOur requirements for construction this model are two things,bistability and coexistence by cell-cell communication
Genetic circuitWe constructed the genetic circuit to fill its requirements. Therefore we made new parts
Our construction parts are Here!!! |
2,WorksApproach:We alternated approach of Wet and Dry.2-0.Wet : Hybrid promoter
2-1.Dry : FormulationWe writed the Formulation 2-2.Wet : AssayWe decided the parameter of hill coefficients by the assay of AHL & IPTG
2-3.Dry : Simulationwet実験で求められたパラメータを用いて、集団が怠け者の細胞のみとなった場合に、一度不安定化して、続いて働き者と怠け者になれることをStocastic Simulationで確認した。このシステムの挙動に必要な他のパラメータ範囲が判明した。
2-4.Wet : AssaySimulationを満たすための他のパラメータが現在のプラスミドで可能であるかを、大腸菌を用いた実験で確認した。
2-5.Future works3,About our team
Advisors: Last year's member
Instructors
Links sunaba |
