Week 16
From 2007.igem.org
(Difference between revisions)
| Line 72: | Line 72: | ||
::'''10/19/07''' | ::'''10/19/07''' | ||
| + | *Transformation: | ||
| + | ::-pLac-GFP-pTet-LacI (on high copy plasmid);:: | ||
| + | ::-pTet-LacI-T-Plambda-RFP; | ||
| + | ::-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp on pSB4A3 (low copy plasmid). | ||
Revision as of 08:46, 23 October 2007
- 10/15/07
- O/N ligations for:
- -I763019;
- -pTet-LacI-T-Plambda-RFP.
- 10/16/07
- Transformation of I763019 and pTet-LacI-T-Plambda-RFP.
- We inoculate:
- -I763005;
- -I763015;
- -I763004.
- Trasformation of I763019, subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.
- 10/17/07
- Miniprep of:
- -I763005;
- -I763015;
- -I763004.
- We inoculate:
- -Ptet-lacI-T-Plambda-RFP-pLac-CI-Lacy-gfp;
- -pTet-LacI-T-pLambda-rfp (high copy).
- Control Digestion for results impagination (X/P):
- - I763020;
- - I763004;
- - I763011;
- - I763007 (on high copy plasmid);
- - I763007 (on low copy plasmid);
- - I763033;
- - I763015;
- - I763012;
- - I763005;
- - I763031.
- 10/18/07
- Miniprep:
- -Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp;
- -pTet-LacI-T-pLambda-rfp (high copy).
- Digestion for:
- -Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp (E/P)
- Band extraction of:
- -Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp (E/P)
- Ligation for:
- -pSB4A3 (E/P)
- -Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp (E/P)
- Ligation for:
- -pLac-GFP(X/P)
- pTetR-LacI (S/P)
- Control Digestion for results impagination:
- - I763016 (low copy plasmid);
- - I763016 (high copy plasmid);
- - I763036;
- - I763019;
- - I763032;
- - I763025 (low copy plasmid);
- - I763025 (high copy plasmid);
- - I763026;
- - I763035.
- 10/19/07
- Transformation:
- -pLac-GFP-pTet-LacI (on high copy plasmid);::
- -pTet-LacI-T-Plambda-RFP;
- -Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp on pSB4A3 (low copy plasmid).
