Week 16
From 2007.igem.org
(Difference between revisions)
| Line 8: | Line 8: | ||
::'''10/16/07''' | ::'''10/16/07''' | ||
| - | *Transformation of [http://partsregistry.org/Part:BBa_I763019 I763019] and I763039. | + | *Transformation of [http://partsregistry.org/Part:BBa_I763019 I763019] and[http://partsregistry.org/Part:BBa_I763039 I763039]. |
*We inoculate: | *We inoculate: | ||
| - | ::-I763005; | + | ::-[http://partsregistry.org/Part:BBa_I763005 I763005]; |
| - | ::-I763015; | + | ::-[http://partsregistry.org/Part:BBa_I763015 I763015]; |
| - | ::-I763004. | + | ::-[http://partsregistry.org/Part:BBa_I763004 I763004]. |
*Trasformation of I763019, subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock. | *Trasformation of I763019, subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock. | ||
| Line 18: | Line 18: | ||
::'''10/17/07''' | ::'''10/17/07''' | ||
*Miniprep of: | *Miniprep of: | ||
| - | ::-I763005; | + | ::-[http://partsregistry.org/Part:BBa_I763005 I763005]; |
| - | ::-I763015; | + | ::-[http://partsregistry.org/Part:BBa_I763015 I763015]; |
| - | ::-I763004. | + | ::-[http://partsregistry.org/Part:BBa_I763004 I763004]. |
*We inoculate: | *We inoculate: | ||
| - | ::-I763029; | + | ::-[http://partsregistry.org/Part:BBa_I763029 I763029]; |
| - | ::-I763039 (high copy). | + | ::-[http://partsregistry.org/Part:BBa_I763039 I763039](high copy). |
*Control Digestion for results impagination (X/P): | *Control Digestion for results impagination (X/P): | ||
| - | ::- I763020; | + | ::-[http://partsregistry.org/Part:BBa_I763020 I763020]; |
| - | ::- I763004; | + | ::-[http://partsregistry.org/Part:BBa_I763004 I763004]; |
| - | ::- I763011; | + | ::-[http://partsregistry.org/Part:BBa_I763011 I763011];; |
| - | ::- I763007 (on high copy plasmid); | + | ::-[http://partsregistry.org/Part:BBa_I763007 I763007](on high copy plasmid); |
| - | ::- I763007 (on low copy plasmid); | + | ::-[http://partsregistry.org/Part:BBa_I763007 I763007] (on low copy plasmid); |
| - | ::- I763033; | + | ::-[http://partsregistry.org/Part:BBa_I763033 I763033]; |
| - | ::- I763015; | + | ::-[http://partsregistry.org/Part:BBa_I763015 I763015]; |
| - | ::- I763012; | + | ::-[http://partsregistry.org/Part:BBa_I763012 I763012]; |
| - | ::- I763005; | + | ::-[http://partsregistry.org/Part:BBa_I763005 I763005]; |
| - | ::- I763031. | + | ::-[http://partsregistry.org/Part:BBa_I763031 I763031]. |
Revision as of 09:25, 23 October 2007
- 10/15/07
- O/N ligations for:
- -[http://partsregistry.org/Part:BBa_I763019 I763019];
- -[http://partsregistry.org/Part:BBa_I763039 I763039].
- 10/16/07
- Transformation of [http://partsregistry.org/Part:BBa_I763019 I763019] and[http://partsregistry.org/Part:BBa_I763039 I763039].
- We inoculate:
- -[http://partsregistry.org/Part:BBa_I763005 I763005];
- -[http://partsregistry.org/Part:BBa_I763015 I763015];
- -[http://partsregistry.org/Part:BBa_I763004 I763004].
- Trasformation of I763019, subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.
- 10/17/07
- Miniprep of:
- -[http://partsregistry.org/Part:BBa_I763005 I763005];
- -[http://partsregistry.org/Part:BBa_I763015 I763015];
- -[http://partsregistry.org/Part:BBa_I763004 I763004].
- We inoculate:
- -[http://partsregistry.org/Part:BBa_I763029 I763029];
- -[http://partsregistry.org/Part:BBa_I763039 I763039](high copy).
- Control Digestion for results impagination (X/P):
- -[http://partsregistry.org/Part:BBa_I763020 I763020];
- -[http://partsregistry.org/Part:BBa_I763004 I763004];
- -[http://partsregistry.org/Part:BBa_I763011 I763011];;
- -[http://partsregistry.org/Part:BBa_I763007 I763007](on high copy plasmid);
- -[http://partsregistry.org/Part:BBa_I763007 I763007] (on low copy plasmid);
- -[http://partsregistry.org/Part:BBa_I763033 I763033];
- -[http://partsregistry.org/Part:BBa_I763015 I763015];
- -[http://partsregistry.org/Part:BBa_I763012 I763012];
- -[http://partsregistry.org/Part:BBa_I763005 I763005];
- -[http://partsregistry.org/Part:BBa_I763031 I763031].
- 10/18/07
- Miniprep:
- -[http://partsregistry.org/Part:BBa_I763029 I763029];
- -[http://partsregistry.org/Part:BBa_I763039 I763039](high copy).
- Digestion for:
- -[http://partsregistry.org/Part:BBa_I763029 I763029] (E/P)
- Band extraction of:
- -[http://partsregistry.org/Part:BBa_I763029 I763029] (E/P)
- Ligation for:
- -[http://partsregistry.org/Part:pSB4A3 pSB4A3] (E/P)
- -[http://partsregistry.org/Part:BBa_I763029 I763029] (E/P)
- Ligation for:
- -[http://partsregistry.org/Part:BBa_I763004 I763004] (X/P)
- -[http://partsregistry.org/Part:BBa_I763026 I763026] (S/P)
- Control Digestion for results impagination:
- - [http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
- - [http://partsregistry.org/Part:BBa_I763016 I763016] (high copy plasmid);
- - [http://partsregistry.org/Part:BBa_I763036 I763036];
- - [http://partsregistry.org/Part:BBa_I763019 I763019];
- - [http://partsregistry.org/Part:BBa_I763032 I763032];
- - [http://partsregistry.org/Part:BBa_I763025 I763025] (low copy plasmid);
- - [http://partsregistry.org/Part:BBa_I763025 I763025] (high copy plasmid);
- - [http://partsregistry.org/Part:BBa_I763026 I763026];
- - [http://partsregistry.org/Part:BBa_I763035 I763035].
- 10/19/07
- Transformation:
- -[http://partsregistry.org/Part:BBa_I763040 I763040] (on high copy plasmid);::
- -[http://partsregistry.org/Part:BBa_I763039 I763039];
- -[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (low copy plasmid).
