Tokyo/Works/Hybrid promoter
From 2007.igem.org
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To realize our model, expression of one part of the genes should be regulated by two factors. Therefore, the promoter upstream of the idlers genetic part was designed to '''sense TWO INPUTS, activated by AHL and repressed by LacI'''. | To realize our model, expression of one part of the genes should be regulated by two factors. Therefore, the promoter upstream of the idlers genetic part was designed to '''sense TWO INPUTS, activated by AHL and repressed by LacI'''. | ||
| - | == | + | ==Design== |
| + | Based on the AHL regulated BioBrick part R0062 and the previous work on LacI regulated promoter (ref. 1), we designed a new promoter regulated by AHL and LacI. Since this promoter is a kind of a '''"HYBRID"''' of the two, we call it '''HYBRID PROMOTER'''. | ||
==実験== | ==実験== | ||
Revision as of 16:03, 23 October 2007
Works top 0. Hybrid promoter 1. Formulation 2. Assay1 3. Simulation 4. Assay2 5. Future works
Purpose
To realize our model, expression of one part of the genes should be regulated by two factors. Therefore, the promoter upstream of the idlers genetic part was designed to sense TWO INPUTS, activated by AHL and repressed by LacI.
Design
Based on the AHL regulated BioBrick part R0062 and the previous work on LacI regulated promoter (ref. 1), we designed a new promoter regulated by AHL and LacI. Since this promoter is a kind of a "HYBRID" of the two, we call it HYBRID PROMOTER.
