From 2007.igem.org

Revision as of 12:26, 24 October 2007 (view source) Hiro (Talk | contribs) (→If Workers are removed...) ← Older edit		Revision as of 12:34, 24 October 2007 (view source) Hiro (Talk | contribs) (→Hybrid promoter) Newer edit →
Line 26:		Line 26:
	==Hybrid promoter==		==Hybrid promoter==
-	これを実装するためにPbはハイブリッドプロモータである必要がある。	+	<!--これを実装するためにPbはハイブリッドプロモータである必要がある。
-	⇒ハイブリッドプロモーターとは？Workのページへ[[Tokyo/Works/Hybrid promoter|Hybrid promoter]]	+	⇒ハイブリッドプロモーターとは？Workのページへ[[Tokyo/Works/Hybrid promoter|hybrid promoter]]-->
		+	To realize such a genetic circuit, Pb should be "'''[[Tokyo/Works/Hybrid promoter|Hybrid promoter]]'''" regulated by AHL and LacI as shown in the figures.
	==他の遺伝子回路の案==		==他の遺伝子回路の案==
	コンセプトモデルを遺伝子回路にする上で、他にもいくつか方法が考えられた。		コンセプトモデルを遺伝子回路にする上で、他にもいくつか方法が考えられた。
	その案を以下に示す。		その案を以下に示す。

---

Revision as of 12:34, 24 October 2007

Abstract　　Concept & Model　　Requirements　　Genetic_circuit　　Works　　About_our_team

Genetic Circuit

Fig.1 is an outline of the genetic circuit in our model, designed to realize our Concept & Model.

All cells, wokers and idlers, have THE SAME genetic circuit (just as 'society' members have practically the same genetic backgrounds).

Workers (State-A)

As shown in Fig. 2, an A-state cell, in which the promoter Pa is "on," expresses the repressor Ra, luxI, and RFP. The repressor Ra "turns off" the promoter Pb and prevents from changing the cell states. This cell also expresses luxI, which makes AHL, cell-cell communication molecules. Downstream of these genes, there is an RFP gene; therefore, we would be able to detect state-A cell with FACS.

Idlers (State-B)

Fig. 3 shows a B-state cell, in which promoter Pb is "turned on" in the presence of AHL, and repressor Rb and GFP are expressed. Repressor Rb represses the expression of promoter Pa and thereby RFP; therefore, fluorescence of GFP is used to detect state-B cells.

If Workers are removed...

As shown in Fig 4, if A-state cells are removed, B-state cells sense the absense of A-cells. hey respond to the decrease in AHL, stop producing repressor Rb. Now that repressions of Pa by Rb as well as that of Pb by Ra are declined, either states cannot be clearly expressed, as if the cells were reset.
(鮎川)toggle-likeという言葉はうちのgenetic circuitには当てはまらないと思います。そもそもtoggle switchにAでもBでもないresetという状態はないのでは？

Hybrid promoter

To realize such a genetic circuit, Pb should be "Hybrid promoter" regulated by AHL and LacI as shown in the figures.

他の遺伝子回路の案

コンセプトモデルを遺伝子回路にする上で、他にもいくつか方法が考えられた。 その案を以下に示す。