UCSF/YeastStrains2
From 2007.igem.org
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| - | 2- Yeast Strains | + | '''2- Yeast Strains''' |
| - | + | : 'Saccharomyces cerevisiae strains: (1) SP163 (W303 MATa, bar1::NatR, far1D, ste2D, ho:: pSte2-Ste2-GFP-RR, mfa2::pCyc-mCherry-PH-ING2, his 3, trp1, leu2), (2) SP164 (W303 MATa, bar1::NatR, far1D, ste2D, ho:: pSte2-Ste2-GFP-HMTM1, mfa2::pCyc-mCherry-PH-ING2, his 3, trp1, leu2),(3) SP165 (W303 MATa, bar1::NatR, far1D, ste2D, ho:: pSte2-Ste2-GFP-WMTM1, mfa2::pCyc-mCherry-PH-ING2, his 3, trp1, leu2). | |
| - | + | :Subsequently, each pRS315-based construct was transformed (selection based on Leu2 marker) into SP163. | |
Revision as of 22:54, 24 October 2007
2- Yeast Strains
- 'Saccharomyces cerevisiae strains: (1) SP163 (W303 MATa, bar1::NatR, far1D, ste2D, ho:: pSte2-Ste2-GFP-RR, mfa2::pCyc-mCherry-PH-ING2, his 3, trp1, leu2), (2) SP164 (W303 MATa, bar1::NatR, far1D, ste2D, ho:: pSte2-Ste2-GFP-HMTM1, mfa2::pCyc-mCherry-PH-ING2, his 3, trp1, leu2),(3) SP165 (W303 MATa, bar1::NatR, far1D, ste2D, ho:: pSte2-Ste2-GFP-WMTM1, mfa2::pCyc-mCherry-PH-ING2, his 3, trp1, leu2).
- Subsequently, each pRS315-based construct was transformed (selection based on Leu2 marker) into SP163.
