Virginia Tech/plasmid construct
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Latest revision as of 03:24, 27 October 2007
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 Our original plan was to build our plasmid in two ways.We wanted to contrast the iGEM method to more traditional methods of molecular biology. Both approaches required three DNA parts:1) A bistable promoter derived from the λ switch (see Designing the Plasmid) 2) Two fluorescent protein genes from the registry 3) A vector backbone from the registry
Our more traditional plan involved synthesizing the plasmid. We knew this would be a quicker way to get the plasmid so that we could begin testing it and obtain experimental data for our model. The results from this approach did not turn out as we expected. We were delayed in finishing the design and having it synthesized.
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