Tokyo/Works/Assay
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== [[Tokyo/AHL assay|1.AHL assay]] == | == [[Tokyo/AHL assay|1.AHL assay]] == | ||
| - | + | '''Purpose''' | |
| - | check how AHL activates lux-lac hybrid promoter. | + | <br>check how AHL activates lux-lac hybrid promoter. |
<br>These parameters(n2,K2) is decided. | <br>These parameters(n2,K2) is decided. | ||
| - | + | '''Result & Conclusion''' | |
| - | Lux-lac hybrid promoter is activated by AHL as shown in fig X （fig番号）. | + | <br>Lux-lac hybrid promoter is activated by AHL as shown in fig X （fig番号）. |
<br>[[Image:hill AHL.JPG|190px]] | <br>[[Image:hill AHL.JPG|190px]] | ||
<br> | <br> | ||
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== [[Tokyo/IPTG assay|2.IPTG assay]] == | == [[Tokyo/IPTG assay|2.IPTG assay]] == | ||
| - | + | '''Purpose''' | |
| - | check how LacI represses this hybrid promoter. In order to adjust LacI concentration, IPTG was added. | + | <br>check how LacI represses this hybrid promoter. In order to adjust LacI concentration, IPTG was added. |
<br>These parameters(n3,K3) is decided. | <br>These parameters(n3,K3) is decided. | ||
<!--<br>To determine the order of the concentration of IPTG necessary for the activation of our lux-lac hybrid promoter in the LacI producing pTrc99A cells. | <!--<br>To determine the order of the concentration of IPTG necessary for the activation of our lux-lac hybrid promoter in the LacI producing pTrc99A cells. | ||
<br> The order of the concentration is used for more detailed assay with narrower range of the IPTG concentration.--> | <br> The order of the concentration is used for more detailed assay with narrower range of the IPTG concentration.--> | ||
| - | + | '''Result & Conclusion''' | |
| - | Lux-lac hybrid promoter is activated by AHL. | + | <br>Lux-lac hybrid promoter is activated by AHL. |
<br>[[Image:hill IPTG.JPG|190px]] | <br>[[Image:hill IPTG.JPG|190px]] | ||
Revision as of 04:02, 25 October 2007
Works top 0.Hybrid promoter 1.Formulation 2.Assay1 3.Simulation 4.Assay2 5.Future works
Purpose of this assay (Hill function fitting)
We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3.
⇒see more details
1.AHL assay
Purpose
check how AHL activates lux-lac hybrid promoter.
These parameters(n2,K2) is decided.
Result & Conclusion
Lux-lac hybrid promoter is activated by AHL as shown in fig X （fig番号）.
⇒see more details
2.IPTG assay
Purpose
check how LacI represses this hybrid promoter. In order to adjust LacI concentration, IPTG was added.
These parameters(n3,K3) is decided.
Result & Conclusion
Lux-lac hybrid promoter is activated by AHL.
⇒see more details
Appendix:Preliminary assays
We conducted preliminary assays to practice experiments and check if the materials and methods worked properly.
