Template:BerkiGEM2007 ArthurSequencingFiles
From 2007.igem.org
(Difference between revisions)
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|AY013||061407||I716002 (pBca9145-yfbE_promote)||D4||G01001||Mixed DNA tube (contamination)||[[BerkiGEM2007-Sequencing-AY013 | AY013]] | |AY013||061407||I716002 (pBca9145-yfbE_promote)||D4||G01001||Mixed DNA tube (contamination)||[[BerkiGEM2007-Sequencing-AY013 | AY013]] | ||
|- | |- | ||
| - | |AY014|| | + | |AY014||061507||I716000 (pBca9145-wbbL)||E1||ca998||long stretch of t's at 696, sequencing got an extra one. Likely read error. Missing a T @ 812, but read quality is low here. Lack of agttgca right after insert?? maybe not??||[[BerkiGEM2007-Sequencing-AY014 | AY014]] |
|- | |- | ||
| - | |AY015|| | + | |AY015||061507||I716000 (pBca9145-wbbL)||E2||ca998||long stretch of t's at 696, sequencing got an extra one. Likely read error. Missing a T at 801. Lack of agttgca right after insert?? maybe not??||[[BerkiGEM2007-Sequencing-AY015 | AY015]] |
|- | |- | ||
|AY016||???||???||???||???||???||[[BerkiGEM2007-Sequencing-AY016 | AY016]] | |AY016||???||???||???||???||???||[[BerkiGEM2007-Sequencing-AY016 | AY016]] | ||
Revision as of 21:35, 17 June 2007
| Sample | Date | Plasmid | Clone | Primer | Result | File Link |
| AY001 | 060407 | pBca9145-Bca1145 | #1 | ca998 | Probably good. Long, ambiguous long AAAAAAA. | AY001 |
| AY002 | 060407 | pBca9145-Bca1145 | #2 | ca998 | Good. | AY002 |
| AY003 | 061207 | pBca9145-HPI/katG (I716???) | A1 | ca998 | super wrong. Seems like only half of HPI/katG, from the AY004.Sal.katG.HPI-Fx "mutate GAT>GAC (BglII)" til the end, was cloned. | AY003 |
| AY004 | 061207 | pBca9145-HPI/katG (I716???) | A3 | ca998 | generally OK. There is one extra G at 584 in the goal plasmid in the calls file. There is low confidence, but I am not sure what to make of it. Possibly should send for recheck at this point; may be necessary to check the mutation point @1308, and the end portion as well. But based on the miniprep digest run yesterday, this guy has a high chance of being the right one. DECISION: sent as AY007 with G01001 for a reverse check. | AY004 |
| AY005 | 061207 | pBca9145-HPI/katG (I716???) | A4 | ca998 | crazy :( there are big gaps | AY005 |
| AY006 | 061207 | pBca9145-wbbL (I716000) | B1 | ca998 | also crazy | AY006 |
| AY007 | 061307 | pBca9145-HPI/katG (I716???) | A3 | G01001 | Looks good actually, only notable thing is silent point mutation @ 1782 TCG > TCA still S. but the mutation point was just missed by sequencing. I don't think it's necessary to recheck; i have faith the primers worked right. well.. idk. major concern still is the possible addition mutation of a G | AY007 |
| AY008 | 061307 | pBca9145-neuS (I716001) | C1 | ca998 | Forward. Looks great! | AY008 |
| AY009 | 061307 | pBca9145-neuS (I716001) | C1 | G01001 | Reverse. Looks great! | AY009 |
| AY010 | 061407 | I716002 (pBca9145-yfbE_promote) | D1 | ca998 | Perfect promoter cloned region. @ 443 there is a deletion of ttgcag. Point mutation @631, T > C | AY010 |
| AY011 | 061407 | I716002 (pBca9145-yfbE_promote) | D1 | G01001 | Mixed DNA tube (contamination) | AY011 |
| AY012 | 061407 | I716002 (pBca9145-yfbE_promote) | D4 | ca998 | Perfect promoter cloned region. @ 443 there is a deletion of ttgcag. Point mutation @631, T > C. @640 addition of a C | AY012 |
| AY013 | 061407 | I716002 (pBca9145-yfbE_promote) | D4 | G01001 | Mixed DNA tube (contamination) | AY013 |
| AY014 | 061507 | I716000 (pBca9145-wbbL) | E1 | ca998 | long stretch of t's at 696, sequencing got an extra one. Likely read error. Missing a T @ 812, but read quality is low here. Lack of agttgca right after insert?? maybe not?? | AY014 |
| AY015 | 061507 | I716000 (pBca9145-wbbL) | E2 | ca998 | long stretch of t's at 696, sequencing got an extra one. Likely read error. Missing a T at 801. Lack of agttgca right after insert?? maybe not?? | AY015 |
| AY016 | ??? | ??? | ??? | ??? | ??? | AY016 |
| AY017 | ??? | ??? | ??? | ??? | ??? | AY017 |
| AY018 | ??? | ??? | ??? | ??? | ??? | AY018 |
| AY019 | ??? | ??? | ??? | ??? | ??? | AY019 |
| AY020 | ??? | ??? | ??? | ??? | ??? | AY020 |
| AY021 | ??? | ??? | ??? | ??? | ??? | AY021 |
| AY022 | ??? | ??? | ??? | ??? | ??? | AY022 |
| AY023 | ??? | ??? | ??? | ??? | ??? | AY023 |
| AY024 | ??? | ??? | ??? | ??? | ??? | AY024 |
| AY025 | ??? | ??? | ??? | ??? | ??? | AY025 |
Start 26
| AY026 | ??? | ??? | ??? | ??? | ??? | AY026 |
| AY027 | ??? | ??? | ??? | ??? | ??? | AY027 |
| AY028 | ??? | ??? | ??? | ??? | ??? | AY028 |
| AY029 | ??? | ??? | ??? | ??? | ??? | AY029 |
| AY030 | ??? | ??? | ??? | ??? | ??? | AY030 |
| AY031 | ??? | ??? | ??? | ??? | ??? | AY031 |
| AY032 | ??? | ??? | ??? | ??? | ??? | AY032 |
| AY033 | ??? | ??? | ??? | ??? | ??? | AY033 |
| AY034 | ??? | ??? | ??? | ??? | ??? | AY034 |
| AY035 | ??? | ??? | ??? | ??? | ??? | AY035 |
| AY036 | ??? | ??? | ??? | ??? | ??? | AY036 |
| AY037 | ??? | ??? | ??? | ??? | ??? | AY037 |
| AY038 | ??? | ??? | ??? | ??? | ??? | AY038 |
| AY039 | ??? | ??? | ??? | ??? | ??? | AY039 |
| AY040 | ??? | ??? | ??? | ??? | ??? | AY040 |
| AY041 | ??? | ??? | ??? | ??? | ??? | AY041 |
| AY042 | ??? | ??? | ??? | ??? | ??? | AY042 |
| AY043 | ??? | ??? | ??? | ??? | ??? | AY043 |
| AY044 | ??? | ??? | ??? | ??? | ??? | AY044 |
| AY045 | ??? | ??? | ??? | ??? | ??? | AY045 |
| AY046 | ??? | ??? | ??? | ??? | ??? | AY046 |
| AY047 | ??? | ??? | ??? | ??? | ??? | AY047 |
| AY048 | ??? | ??? | ??? | ??? | ??? | AY048 |
| AY049 | ??? | ??? | ??? | ??? | ??? | AY049 |
| AY050 | ??? | ??? | ??? | ??? | ??? | AY050 |
