Freiburg07/In vivo test I

From 2007.igem.org

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<p>With this concentration of Ca2+, Amp and IPTG were following plates pour.</p>
<p>With this concentration of Ca2+, Amp and IPTG were following plates pour.</p>
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[https://2007.igem.org/Freiburg BACK]
<p>3 plates IPTG</p>
<p>3 plates IPTG</p>
<p>3 plates IPTG + AMP</p>
<p>3 plates IPTG + AMP</p>

Revision as of 13:11, 25 October 2007

In vivo test I

Ca2+ stock:        100mM

Ca2+  [µg/ml] 0 0.1 1.0 10
IPTG [µg/ml] 0.5 0.5 0.5 0.5
Amp [µg/ml] 50 50 50 50

With this concentration of Ca2+, Amp and IPTG were following plates pour.


BACK

3 plates IPTG

3 plates IPTG + AMP

3 plates IPTG + Amp + Ca2+ (0.1)

3 plates IPTG + Amp + Ca2+ (1.0)

3 plates IPTG + Amp + Ca2+ (10)

3 plates IPTG + Ca2+ (0.1)

3 plates IPTG + Ca2+ (1.0)

3 plates IPTG + Ca2+ (10)

500 cells/100µl were plated in each plate.

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