USTC

Our Project:
Extensible Logic Circuit in Bacteria

Artificial Bio-Logic Circuit is composed of "logic gates" and "wires" like Digital Electronic Circuits. Though we have been enjoying the advantages of ultra-large-scale electronic circuits in modern life, we still cannot implement a somewhat small-scale circuit in vivo with several levels of gates.

Our project is to provide a new method for building up a fully extensible bio-logic circuit in bacteria. A small piece of DNA containing cis-acting elements and favored for its small scale and potential to implement complicated logic computation in vivo, can be systematically built up and act as a gate. Meanwhile, artificial repressors with highly-specified DNA-recognition regions are able to transmit signals without mutual interference, just as copper wires. In this way, a circuit can be constructed regardless of the number of logic gates and the manner of connections between the wires.

A demonstration system has also been assembled to show the practicality of this method.Just like Digital Electronic Circuits in early days, it is simple and ugly. But how will it appear in future?

From left to right
Back row: Zhao Yun, Liu ZQ, Ma XY
Front row: Ma Rui, Su XF, Ding Bo, Zhan Jian

Project

For detailed description, please click each hyperlink below.

Introduction

Core Logic Components:

Logic-Gate Promoters

Wires without Interference:

Repressor Evolution on Plates

Repressor Evolution in Silico

Peripheral Devices:

Inputs and Outputs

Extensible System:

A Demonstration

Further More

Statistics

In less than 5 months, our

Cosumption

4mL LB medium consumption
> 1400 tubes
Competence Strain we've made
~ 300 EP tubes
96-Well Plates we've used
~ 10 plates

Detection

Colony-PCR detection
> 500 times
Fluorescence detection
> 100 times
DNA sequencing
~ 130 times

Measurement

Fluorescent Density measuring
> 200 times
ONPG assay
~ 70 times

Members

Resources

Modeling Utilities

Protocols

Sponsors

Service Providers

Gallery

Team Photos

Laboratory

[http://sg.ustc.edu.cn/mediawiki/index.php/IGEM_USTC_2007:USTCPhotos USTC Photos]

[http://sg.ustc.edu.cn/mediawiki/index.php/IGEM_USTC_2007:HuangshanPhotos Huangshan Mountain]

Links

[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=USTC Parts Made by USTC iGEM 2007]

[http://partsregistry.org iGEM Standard Parts' Catalog ]

IGEM2007 Team List

[http://www.ustc.edu.cn Univ. of Sci. and Tech. of China ]