BerkiGEM2007-SamanthaConstructionFile9
From 2007.igem.org
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'''[rbs][Cre]''' <br> | '''[rbs][Cre]''' <br> | ||
'''rbs library - want a weak rbs actually''' <br> | '''rbs library - want a weak rbs actually''' <br> | ||
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Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small) <br> | Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small) <br> | ||
Digest pBca9145-Bca1117 (EcoRI/BglII, 3101+9bp, Large)<br> | Digest pBca9145-Bca1117 (EcoRI/BglII, 3101+9bp, Large)<br> | ||
| - | Plate on CamAmp | + | Plate on CamAmp <br> |
Product is pBca9145-I716224. <br> | Product is pBca9145-I716224. <br> | ||
Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small) <br> | Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small) <br> | ||
Digest pBca9145-Bca1117 (EcoRI/BglII, 3101+9bp, Large) <br> | Digest pBca9145-Bca1117 (EcoRI/BglII, 3101+9bp, Large) <br> | ||
| - | Plate on KanAmp | + | Plate on KanAmp<br> |
Product is pBca9145-I716225. <br> | Product is pBca9145-I716225. <br> | ||
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Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small) <br> | Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small) <br> | ||
Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large)<br> | Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large)<br> | ||
| - | Plate on CamAmp | + | Plate on CamAmp <br> |
| - | Product is pBca9145- | + | Product is pBca9145-I716226. <br> |
Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small) <br> | Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small) <br> | ||
Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large) <br> | Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large) <br> | ||
| - | Plate on KanAmp | + | Plate on KanAmp <br> |
| - | Product is pBca9145- | + | Product is pBca9145-I716227. <br> |
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Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small) <br> | Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small) <br> | ||
Digest pBca9145-I716213 (EcoRI/BglII, 3101+9bp, Large)<br> | Digest pBca9145-I716213 (EcoRI/BglII, 3101+9bp, Large)<br> | ||
| - | Plate on CamAmp | + | Plate on CamAmp <br> |
| - | Product is pBca9145- | + | Product is pBca9145-I716228. <br> |
Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small) <br> | Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small) <br> | ||
Digest pBca9145-I716213 (EcoRI/BglII, 3101+9bp, Large) <br> | Digest pBca9145-I716213 (EcoRI/BglII, 3101+9bp, Large) <br> | ||
| - | Plate on KanAmp | + | Plate on KanAmp <br> |
| - | Product is pBca9145- | + | Product is pBca9145-I716229. <br> |
Revision as of 19:00, 20 June 2007
[rbs][Cre]
rbs library - want a weak rbs actually
3 Different Cre variants (ATG Bca1117, GTG I716212 and TTG I716213)
ATG Cre variants
Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-Bca1117 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp
Product is pBca9145-I716224.
Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small)
Digest pBca9145-Bca1117 (EcoRI/BglII, 3101+9bp, Large)
Plate on KanAmp
Product is pBca9145-I716225.
GTG Cre variants
Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp
Product is pBca9145-I716226.
Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small)
Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large)
Plate on KanAmp
Product is pBca9145-I716227.
TTG Cre variants
Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-I716213 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp
Product is pBca9145-I716228.
Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small)
Digest pBca9145-I716213 (EcoRI/BglII, 3101+9bp, Large)
Plate on KanAmp
Product is pBca9145-I716229.
