BerkiGEM2007-AustinConstructionFile23
From 2007.igem.org
(Difference between revisions)
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PCR ca1099F/AD0005R on 9145-I716053 -HbA alpha 2X mutant-<br> | PCR ca1099F/AD0005R on 9145-I716053 -HbA alpha 2X mutant-<br> | ||
PCR AD0006F/ca1099R on 9145-I716053 –HbA alpha 2X mutant-<br> | PCR AD0006F/ca1099R on 9145-I716053 –HbA alpha 2X mutant-<br> | ||
- | Digest both with BsaI/DpnI, and Ligate together Product is 9145- | + | Digest both with BsaI/DpnI, and Ligate together Product is 9145-I716073<br> |
Revision as of 21:48, 26 June 2007
PCR ca1099F/AD0005R on 9145-I716053 -HbA alpha 2X mutant-
PCR AD0006F/ca1099R on 9145-I716053 –HbA alpha 2X mutant-
Digest both with BsaI/DpnI, and Ligate together Product is 9145-I716073