Chiba/Communication
From 2007.igem.org
(→Our Aim) |
|||
| Line 11: | Line 11: | ||
==Communication Unit== | ==Communication Unit== | ||
===Our Aim=== | ===Our Aim=== | ||
| - | [[Image:Chiba_prj_com.png|frame|''' | + | [[Image:Chiba_prj_com.png|frame|'''Fig13.''' Quorum sensing for activating receivers!]] |
Our aim is to make bacteria stick around a bacteria core. | Our aim is to make bacteria stick around a bacteria core. | ||
Make a sender, a bacteria core that sends a signal, and a receiver, a bacteria that receive a signal. | Make a sender, a bacteria core that sends a signal, and a receiver, a bacteria that receive a signal. | ||
Revision as of 05:04, 27 October 2007
|
Introduction | Project Design ( 1.Affinity Tag | 2.Communication Module | 3.Size Control ) | Making Marimos | Our Goal |
Communication Unit
Our Aim
Our aim is to make bacteria stick around a bacteria core.
Make a sender, a bacteria core that sends a signal, and a receiver, a bacteria that receive a signal.
Our system works like this: Sender core, which is sticking each other in advance, sends a signal to receivers, which grow stickey tags.
Then they stick together. We use Quorum sensing as a signal.
About Quorum Sensing
Quorum sensing is a cell-to-cell signaling action of bacteria. They detect the cell density of the same species and coordinate the expression behavior of their cells. Species of Gram-Negative signaling transfer molecules (so-called autoinducer) is a series of acyl homoserine lactone (AHL). When the AHL concentration exceeds a threshold, that is, the cell population is over a certain number, the promoter plux transcription is activated.
Detailed gene circuit is discribed in Fig. 2.
- more about quorum sensing
[http://partsregistry.org/Featured_Parts:Cell-Cell-Signaling Cell-Cell-Signaling]
[http://www.che.caltech.edu/groups/fha/quorum.html About Quorum sensing]
Experiments: New Parts!!
Recievers
New Parts We Made!!
- AHL > GFP generator (constitutive aiiA) : [http://partsregistry.org/Part:BBa_I729006 BBa_T729006]
- AHL > GFP & aiiA generator : [http://partsregistry.org/Part:BBa_I729005 BBa_I729005]
- Sensitive AHL > GFP generator : [http://partsregistry.org/Part:BBa_I729004 BBa_I729004]
From Biobrick
- BBa_T9002
Future Work
- inverter-aiiA
Senders
New Parts We Made
- MetK Sender ( could not deposit to registry )
From Biobrick
- Sender(BBa_S03623)
