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Revision as of 02:13, 2 July 2007 (view source) AustinDay (Talk | contribs) ← Older edit		Revision as of 21:49, 2 July 2007 (view source) AustinDay (Talk | contribs) Newer edit →
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-	Beta portion<br>	+	Alpha: <br>
-	pcr CA1154F/G00101 on 9145-I716072 (575 bp)<br>	+	PCR CA1150/CA1099R on I716070 (1727 Bp)<br>
-	Product is ADtemp1<br>	+	Digest (Bsa1/Bgl2) and ligate with beta portion.<br> <br>
-	Sub ADtemp1 into pTRC plasmid with (Bgl2/XhoI)<br>	+
-	Product is ADtemp2<br>	+	Beta: <br>
-	pcr CA1099R/D58 on ADtemp2 (3465 bp)<br>	+
-	Product is ADtemp3<br>	+	PCR CA1099R/D58 on Part Ptrc-I716080 <br>
-	_______________________________________________________<br>	+	Digest (Bsa1/BamH1) and ligate with alpha portion. <br>
-	Alpha portion<br>	+	<br><br>
-	Pcr CA1150F/CA1099R on I716077 ()<br>	+	Product is pTRC-I716074 (best yield)
-	Product is ADtemp5<br>	+
-	____________________________________________________________<br>	+
-	Digest ADtemp3 (BsaI/BamHI)<br>	+
-	Digest ADtemp5 (BsaI/BglII)<br>	+
-	Ligate, product is: I716074 (Best yield)	+
-	<br>	+

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Revision as of 21:49, 2 July 2007

Alpha:
PCR CA1150/CA1099R on I716070 (1727 Bp)
Digest (Bsa1/Bgl2) and ligate with beta portion.

Beta:

PCR CA1099R/D58 on Part Ptrc-I716080
Digest (Bsa1/BamH1) and ligate with alpha portion.


Product is pTRC-I716074 (best yield)