BerkiGEM2007-AustinConstructionFile24
From 2007.igem.org
(Difference between revisions)
| Line 6: | Line 6: | ||
PCR CA1099F/G00101 on Part 9145-I716081 <br> | PCR CA1099F/G00101 on Part 9145-I716081 <br> | ||
| - | Digest (Bsa1/BamH1) and ligate with alpha portion. <br> | + | Digest (Bsa1/BamH1) (1447/small, Larger) and ligate with alpha portion. <br> |
<br><br> | <br><br> | ||
Product is 9145-I716074 (best yield) | Product is 9145-I716074 (best yield) | ||
Revision as of 18:28, 5 July 2007
Alpha:
PCR CA1150/CA1099R on 9145-I716077 (1727 Bp)
Digest (Bsa1/Bgl2) and ligate with beta portion.
Beta:
PCR CA1099F/G00101 on Part 9145-I716081
Digest (Bsa1/BamH1) (1447/small, Larger) and ligate with alpha portion.
Product is 9145-I716074 (best yield)
