Melbourne/IGEM 2007 kit
From 2007.igem.org
(Difference between revisions)
| Line 8: | Line 8: | ||
====Method to resuspend DNA==== | ====Method to resuspend DNA==== | ||
| - | + | #Punctured foil with pipette tip clearing opening to well without touching bottom of well. | |
| + | #Resuspended well contents in 15uL milliQ water. | ||
| + | #transfere to epindorf tube. | ||
| + | #Lable epindorf in red for Ampicillan resistance and blue for kanamycin resistance | ||
| + | ##Format P<plate number> _ <column number><row character> e.g. P1 14H | ||
| + | #Mark lid Tab with Red or Blue for drug resistance as above. | ||
| + | #Stored epindorf at -20. | ||
====Lab Location==== | ====Lab Location==== | ||
| - | *Location Fridge -4deg Igem Box | + | *Location kit plates & additional part(BBa_I15010) epindorf: Fridge -4deg Igem Box |
| + | *Location additional part (BBa_I15010) | ||
*Physical apearance: 4 by 96well plates covered in foil, notches on bottom corners. | *Physical apearance: 4 by 96well plates covered in foil, notches on bottom corners. | ||
| + | |||
| + | *Loacation of DNA from opened wells: Freezer -20deg Igem Box 1 | ||
| + | *Physical apearance: pinkish fluid in ependorfs labled as above. | ||
| + | |||
*Permision to use required? No | *Permision to use required? No | ||
====Supplier:==== | ====Supplier:==== | ||
| - | *Supplier: | + | *Supplier:MIT |
*Suppliers part no: | *Suppliers part no: | ||
| - | *Contact details for supplier: | + | *Contact details for supplier: Meagan Lizarazo |
| - | *Email: | + | **MIT CSAIL |
| + | **32 Vassar Street, Room 32-314 | ||
| + | **Cambridge, MA 02139 | ||
| + | *Email:meaganl@mit.edu | ||
| + | *Gmail Chat at: meagan.lizarazo@gmail.com | ||
*Phone: | *Phone: | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | Send any iGEM related correspondence to: meaganl AT mit DOT edu | ||
| + | |||
| + | Contact me via Gmail Chat at: meagan DOT lizarazo AT gmail DOT com | ||
Revision as of 02:08, 5 July 2007
<return to list of primary reagents> <team home page>
Contents |
Physical data
- Description:
IGEM 2007 KIT
- Data in IGEM 2007 registery
- Approximate cost: $3000.00 USD/kit
Method to resuspend DNA
- Punctured foil with pipette tip clearing opening to well without touching bottom of well.
- Resuspended well contents in 15uL milliQ water.
- transfere to epindorf tube.
- Lable epindorf in red for Ampicillan resistance and blue for kanamycin resistance
- Format P<plate number> _ <column number><row character> e.g. P1 14H
- Mark lid Tab with Red or Blue for drug resistance as above.
- Stored epindorf at -20.
Lab Location
- Location kit plates & additional part(BBa_I15010) epindorf: Fridge -4deg Igem Box
- Location additional part (BBa_I15010)
- Physical apearance: 4 by 96well plates covered in foil, notches on bottom corners.
- Loacation of DNA from opened wells: Freezer -20deg Igem Box 1
- Physical apearance: pinkish fluid in ependorfs labled as above.
- Permision to use required? No
Supplier:
- Supplier:MIT
- Suppliers part no:
- Contact details for supplier: Meagan Lizarazo
- MIT CSAIL
- 32 Vassar Street, Room 32-314
- Cambridge, MA 02139
- Email:meaganl@mit.edu
- Gmail Chat at: meagan.lizarazo@gmail.com
- Phone:
Send any iGEM related correspondence to: meaganl AT mit DOT edu
Contact me via Gmail Chat at: meagan DOT lizarazo AT gmail DOT com
- PhillipDodson 03:48, 1 July 2007 (EDT):Primary Reagent Template 1/July/2007
