BerkiGEM2007-AustinConstructionFile24
From 2007.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
Alpha: <br> | Alpha: <br> | ||
PCR CA1150/CA1099R on 9145-I716077 (1727 Bp)<br> | PCR CA1150/CA1099R on 9145-I716077 (1727 Bp)<br> | ||
| - | Digest (Bsa1/Bgl2) and ligate with beta portion.<br> <br> | + | Digest (Bsa1/Bgl2) (~2000/Small, Larger) and ligate with beta portion.<br> <br> |
Beta: <br> | Beta: <br> | ||
Revision as of 18:30, 5 July 2007
Alpha:
PCR CA1150/CA1099R on 9145-I716077 (1727 Bp)
Digest (Bsa1/Bgl2) (~2000/Small, Larger) and ligate with beta portion.
Beta:
PCR CA1099F/G00101 on Part 9145-I716081
Digest (Bsa1/BamH1) (1447/small, Larger) and ligate with alpha portion.
Product is 9145-I716074 (best yield)
