Paris/July 6
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| - | Preparing growth medium for transduction screening | + | == Preparing growth medium for transduction screening == |
| - | + | If the transduction works (i.e. an homologous recombinaison that should delete DapA by inserting an erythromycin resistant cassette), transducted MG1655 Coli should grow in a medium LB+erythro+ DAP but can't grow in a medium LB+erythro without DAP. | |
*Preparation of DAP solution from the powder (50mM). See [[Paris/PROTOCOLS | Protocols]] | *Preparation of DAP solution from the powder (50mM). See [[Paris/PROTOCOLS | Protocols]] | ||
Revision as of 21:18, 16 July 2007
Preparing growth medium for transduction screening
If the transduction works (i.e. an homologous recombinaison that should delete DapA by inserting an erythromycin resistant cassette), transducted MG1655 Coli should grow in a medium LB+erythro+ DAP but can't grow in a medium LB+erythro without DAP.
- Preparation of DAP solution from the powder (50mM). See Protocols
- Making 10 petri dish (LB+tet+citrate+DAP). See Protocols
- Making 10 petri dish (LB+erythromycin+citrate+DAP). See Protocols
