From 2007.igem.org

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	===Enzymes===		===Enzymes===
	*[[Melbourne/primary Restriction enzymes]]		*[[Melbourne/primary Restriction enzymes]]
-	** NotI,
-	**EcoRI,
-	**XbaI,
-	**HindIII,
-	**SpeI,
	*[[Melbourne/primary Ligase|Ligase]]		*[[Melbourne/primary Ligase|Ligase]]
	*[[Melbourne/primary competant DH5a|DH5a Competent cells]]		*[[Melbourne/primary competant DH5a|DH5a Competent cells]]

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Revision as of 07:07, 11 July 2007

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Contents 1 Primary Reagents 1.1 Kits 1.2 Enzymes 1.3 Antibiotics 1.4 Chemicals 1.5 Other 2 Disposables 2.1 TUBES:

Primary Reagents

Kits

• IGEM 2007 kit
• Miniprep kit
• Gel clean up kit
• Site directed mutagenesis (stratagene)
  • Quickchange multi 200515(x10),200514(x30): 3-5 changes 1 primer each 50% <8Kb
  • Quickchange 200518(x30) 200519(x10): single change 2 primers 90% <8Kb
  • QuickchangeII XL 200522(x30) 200521(x10): single change 2 primers 80% >8Kb
• Primer design program : http://www.stratagene.com/sdmdesigner/default.aspx

Enzymes

• Melbourne/primary Restriction enzymes
• Ligase
• DH5a Competent cells
• DNA ladder

Antibiotics

• Ampicilian
• Kanamycin

Chemicals

• Agar
• Agarose
• Ethidium Bromide
• EDTA
• Glycerol
• Loading dye for DNA gel
• NaCl
• TRIS

Other

• DNA Loading Dye
• ice
• milliQ water
• Distilled water

Disposables

• Parrafilm
• Tips (10, 200, 1000 ul)
• Gloves (Large , Med, Small)
• Scalpels
• Tube racks
• Freezer boxes

TUBES:

• Falcon: 10ml, 50ml
• microcentrifuge: 1.7ml
• PCR