Paris/Oligos
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* Lox71-FtsZ: | * Lox71-FtsZ: | ||
| - | Lox71-FtsZ-F | + | Lox71-FtsZ-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT 89 |
| - | TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT 89 | + | |
| - | FtsZ-R | + | FtsZ-R TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCAGGAAT 49 |
| - | TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCAGGAAT 49 | + | |
* Lox71-FtsA-FtsZ: | * Lox71-FtsA-FtsZ: | ||
Revision as of 10:07, 13 July 2007
Constructions
- Lox71-FtsZ:
Lox71-FtsZ-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT 89
FtsZ-R TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCAGGAAT 49
- Lox71-FtsA-FtsZ:
Lox71-FtsA-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAATCAGGCACAGGCAGAACAACAATG 89
- Deletion of Ecor1 site in FtsZ:
DEcoR1-FtsZ-R CGGTATTTACCGCGAAGAACTCAACACCTTCAATGCGCTC 40
DEcoR1-FtsZ-F TGCGCGAGCGCATTGAAGGTGTTGAGTTCTTCGCGGTAAA 40
- Lox66-DapAColi:
Lox66-DapAColi-F TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAGTTTGCACAGAGGATGGCCCATGTT 84
DapAColi-R TTTCTGCAGCGGCCGCTACTAGTATTATTACAGCAAACCGGCATGCTTAAGC 52
- Lox66-DapASubtilis:
Lox66-DapASubtilis-F TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAATCATGATGAGGTGAATAAAATGAA 84
DapASubtilis-R TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT 52
- Lox71-KmR-Lox66:
Lox71-KmR-F TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACGAAGTTATCTGATCCTTCAACTCAGCAAAAGTTC 85
Lox66-KmR-R TTTTACTAGTAGCGGCCGCTGCAGTACCGTTCGTATAATGTATGCTATACCAAGTTATTTATTAGAAAAACTCATCGAGCATCAAA 86
- Deletion of Pst1 site in DGAT:
DPst1-DGAT-F AGTGCTGTCGTATATGGCCCAGCAGGACTCAACATAATTT 40
DPst1-DGAT-R AAATTATGTTGAGTCCTGCTGGGCCATATACGACAGCACT 40
