Boston University/Bacterial Conjugation Results
From 2007.igem.org
(Difference between revisions)
(→Filter conjugation to transfer the plasmid pMS291(lacZ) from E.coli SM10 to S. oneidensis AS-92) |
(→Filter conjugation to transfer the plasmid pMS291(lacZ) from E.coli SM10 to S. oneidensis AS-92) |
||
| Line 10: | Line 10: | ||
Plate 1: 7 separate conjugation mixes were plated. The white is the filter upon which the bacteria conjugate. S. oneidensis is red. | Plate 1: 7 separate conjugation mixes were plated. The white is the filter upon which the bacteria conjugate. S. oneidensis is red. | ||
| + | |||
[[Image:BU_conjugation2.jpg]] | [[Image:BU_conjugation2.jpg]] | ||
| - | Plate 2 | + | Plate 2 |
| + | |||
[[Image:BU_conjugation3.jpg]] | [[Image:BU_conjugation3.jpg]] | ||
| - | Plate 3 | + | Plate 3 |
| + | |||
[[Image:BU_conjugation4.jpg]] | [[Image:BU_conjugation4.jpg]] | ||
| - | Plate 4 | + | Plate 4 |
| + | |||
[[Image:BU_conjugation5.jpg]] | [[Image:BU_conjugation5.jpg]] | ||
| - | Plate 5 | + | Plate 5 |
[[Boston University | Back]] | [[Boston University | Back]] | ||
Revision as of 16:28, 16 July 2007
Filter conjugation to transfer the plasmid pMS291(lacZ) from E.coli SM10 to S. oneidensis AS-92
- The plasmid pMS291 contained a kanamycin resistance gene. S. oneidensis has a native gentamicin resistance.
- The plasmid pMS291 is first transformed into E.coli SM10 and then the conjugation protocol is run to encourage conjugal transfer of the plasmid to S. oneidensis AS-92. A double-antibiotic selection method is used to select for successful S. onedensis transformants which have a resistance for both kanamycin and gentamicin.
Plate 1: 7 separate conjugation mixes were plated. The white is the filter upon which the bacteria conjugate. S. oneidensis is red.
Plate 2
Plate 3
Plate 4
Plate 5
