Paris/July 6
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*Making 10 petri dish (LB+erythromycin+citrate+DAP). See [[Paris/PROTOCOLS#Preparing growth media | Protocols]] | *Making 10 petri dish (LB+erythromycin+citrate+DAP). See [[Paris/PROTOCOLS#Preparing growth media | Protocols]] | ||
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| + | == Acinetobacter and E.Coli on LNMM Nile Red solid culture == | ||
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| + | Nile Red can be excited by light around 312nm (Spiekermann,1999). | ||
| + | After 24h incubation, we observed Acinetobacter and E.Coli on LNMM with and without Nile Red: | ||
[[Paris|<<home]] | [[Paris|<<home]] | ||
Revision as of 13:24, 25 July 2007
Preparing growth medium for transduction screening
If the transduction works (i.e. an homologous recombinaison that should delete DapA by inserting an erythromycin resistant cassette), transducted MG1655 Coli should grow in a medium LB+erythro+ DAP but can't grow in a medium LB+erythro without DAP.
- Preparation of DAP solution from the powder (50mM). See Protocols
- Making 10 petri dish (LB+tet+citrate+DAP). See Protocols
- Making 10 petri dish (LB+erythromycin+citrate+DAP). See Protocols
Acinetobacter and E.Coli on LNMM Nile Red solid culture
Nile Red can be excited by light around 312nm (Spiekermann,1999). After 24h incubation, we observed Acinetobacter and E.Coli on LNMM with and without Nile Red:
