From 2007.igem.org

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Revision as of 17:23, 19 July 2007

== Transforming BioBricks (Maija)

1. 9 reactions including 5 tubes of competent cells (4 x Top 10, 1 x DB3.1).
2. split the content (~75ul) in two.
3. competent cells (~33ul) into eppendorfs on ice..
4. add 1ul of plasmid DNA.
5. incubate on ice for at least 5 mins.
6. heat shock +42 ºC for 45 secs.
7. back on ice for 2 mins to reduce damages.
8. add 1ml of soc media.
9. incubate +37ºC in a shaker for 1 hour.
10. spread the reaction on kan/carb LB plates.
11. grow +37 ºC overnight.