Wisconsin/Protocol:Cell Culture
From 2007.igem.org
< Wisconsin(Difference between revisions)
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Using sterile technique: | Using sterile technique: | ||
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1. Load 4mL of LB+Amp broth into culture tubes | 1. Load 4mL of LB+Amp broth into culture tubes | ||
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2. Use sterile stick and dab the desired bacterial colony from your plate | 2. Use sterile stick and dab the desired bacterial colony from your plate | ||
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3. Swirl stick in culture tube, ~5 sec. | 3. Swirl stick in culture tube, ~5 sec. | ||
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4. Flame culture tube before closing | 4. Flame culture tube before closing | ||
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5. Place culture tube in 37C shaker for 18-20 hrs. | 5. Place culture tube in 37C shaker for 18-20 hrs. | ||
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Flame stick container and LB+Amp broth flask when done. | Flame stick container and LB+Amp broth flask when done. | ||
Latest revision as of 17:29, 19 July 2007
Using sterile technique:
1. Load 4mL of LB+Amp broth into culture tubes
2. Use sterile stick and dab the desired bacterial colony from your plate
3. Swirl stick in culture tube, ~5 sec.
4. Flame culture tube before closing
5. Place culture tube in 37C shaker for 18-20 hrs.
Flame stick container and LB+Amp broth flask when done.
