Glasgow/Wetlab/Orders

From 2007.igem.org

(Difference between revisions)
(→Emma's primers)
Line 7: Line 7:
!Tm
!Tm
|-
|-
-
|*PhZ-Up
+
|*(***)-Up
-
|5' TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3'
+
|5' *** C 3'
|65.6
|65.6
|-
|-
-
|*PhZ-Low
+
|*(***)-Low
-
|5' CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CA G 3'
+
|5' *** 3'
|64.8
|64.8
|-
|-
-
|*Bbp_PhZ_Up
+
|*Bbp_(***)_Up
-
|5' gaa ttc gcg gcc gct tct aga TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3'
+
|5' *** 3'
|63.6
|63.6
|-
|-
-
|*Bbs_PhZ_Low
+
|*Bbs_(***)_Low
-
|5' ctg cag cgg ccg cta cta gta CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CAG 3'
+
|5' *** 3'
|64.2
|64.2
|-
|-
Line 32: Line 32:
|-
|-
|*Methyl_1
|*Methyl_1
-
|5' TTT TTG AAT TCT TTC GGA CGC AGG AAA AG 3'
+
|5' *** 3'
|66.3
|66.3
|-
|-
|*Methyl_2
|*Methyl_2
-
|5' TTT TTG GAT CCG TTG AAA GTT CCG ATT CA 3'
+
|5' *** 3'
|66.2
|66.2
|-
|-
|*Bbp_Methyl_1
|*Bbp_Methyl_1
-
|5' gaa ttc gcg gcc gct tct aga TTT TTG AAT TCT TTC GGA CGC AGG AAA AG 3'
+
|5' *** 3'
|63.9
|63.9
|-
|-
|*Bbs_Methyl_2
|*Bbs_Methyl_2
-
|5' ctg cag cgg ccg cta cta gta TTT TTG GAT CCG TTG AAA GTT CCG ATT CA 3'
+
|5' *** 3'
|66.9
|66.9
|-
|-
-
|Phz_M_for_1
+
|(***)_M_for_1
-
|5' atg aat aat tcg aat ctt gct gct 3'
+
|5' *** 3'
|65.4
|65.4
|-
|-
-
|Phz_M_rev_1
+
|(***)_M_rev_1
-
|5' tca ggc cct ggc agc gac 3'
+
|5' *** 3'
|60.3
|60.3
|-
|-
-
|Bbp_Phz_M_for_1
+
|Bbp_(***)_M_for_1
-
|5' gaa ttc gcg gcc gct tct aga tga ata att cga atc ttg ctg ct 3'
+
|5' *** 3'
|64.5
|64.5
|-
|-
-
|Bbs_Phz_M_rev_1
+
|Bbs_(***)_M_rev_1
-
|5' ctg cag cgg ccg cta cta gta tca ggc cct ggc agc gac 3'
+
|5' *** 3'
|60.3
|60.3
|-
|-
|*Oxy_1
|*Oxy_1
-
|5' TCG AAC ACT CTA GAA AAG GAA GCA CC 3'
+
|5' *** 3'
|60.2
|60.2
|-
|-
|*Oxy_2
|*Oxy_2
-
|5' TTT TTT GCA TGC TAG CGT GGC CGT TCC 3'
+
|5' *** 3'
|59.2
|59.2
|-
|-
|*Bbp_Oxy_1
|*Bbp_Oxy_1
-
|5' gaa ttc gcg gcc gct tct aga TCG AAC ACT CTA GAA AAG GAA GCA CC 3'
+
|5' *** 3'
|65.1
|65.1
|-
|-
|*Bbs_Oxy_2
|*Bbs_Oxy_2
-
|5' ctg cag cgg ccg cta cta gta TTT TTT GCA TGC TAG CGT GGC CGT TCC 3'
+
|5' *** 3'
|66.9
|66.9
|-
|-
-
|Phz_S_for_1
+
|(***)_S_for_1
-
|5' atg agc gaa ccc atc gat at 3'
+
|5' *** 3'
|71.2
|71.2
|-
|-
-
|Phz_S_rev_1
+
|(***)_S_rev_1
-
|5' cta gcg tgg ccg ttc cac ctg 3'
+
|5' *** 3'
|60.2
|60.2
|-
|-
-
|Bbp_Phz_S_for_1
+
|Bbp_(***)_S_for_1
-
|5' gaa ttc gcg gcc gct tct aga tga gcg aac cca tcg ata t 3'
+
|5' *** 3'
|70.9
|70.9
|-
|-
-
|Bbs_Phz_S_rev_1
+
|Bbs_(***)_S_rev_1
-
|5' ctg cag cgg ccg cta cta gta cta gcg tgg ccg ttc cac ctg 3'
+
|5' *** 3'
|63.3
|63.3
|-
|-
-
|Phz_H_up
+
|(***)_H_up
-
|5' CGC ACG GAT CCT TTC AGA ATG TTC 3'
+
|5' *** 3'
|69.9
|69.9
|-
|-
-
|Phz_H_low
+
|(***)_H_low
-
|5' GCC ACG CCA AGC TTC ACG CTC A 3'
+
|5' *** 3'
|70.6
|70.6
|-
|-
-
|Bbp_Phz_Up
+
|Bbp_(***)_Up
-
|5' gaa ttc gcg gcc gct tct aga TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3'
+
|5' *** 3'
|63.3
|63.3
|-
|-
-
|Bbs_Phz_Low
+
|Bbs_(***)_Low
-
|5' ctg cag cgg ccg cta cta gta CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CAG 3'
+
|5' *** 3'
|57.7
|57.7
|}
|}
-
 
-
Primers marked with * are taken from previous studies.
 

Revision as of 14:19, 24 July 2007

Glasgow Main Page

Emma's primers

Name Sequence Tm
*(***)-Up 5' *** C 3' 65.6
*(***)-Low 5' *** 3' 64.8
*Bbp_(***)_Up 5' *** 3' 63.6
*Bbs_(***)_Low 5' *** 3' 64.2
Bbp_Gene 5' gaa ttc gcg gcc gct tct ag 3' 65.7
Bbs 5' ctg cag cgg ccg cta cta gta 3' 65.3
*Methyl_1 5' *** 3' 66.3
*Methyl_2 5' *** 3' 66.2
*Bbp_Methyl_1 5' *** 3' 63.9
*Bbs_Methyl_2 5' *** 3' 66.9
(***)_M_for_1 5' *** 3' 65.4
(***)_M_rev_1 5' *** 3' 60.3
Bbp_(***)_M_for_1 5' *** 3' 64.5
Bbs_(***)_M_rev_1 5' *** 3' 60.3
*Oxy_1 5' *** 3' 60.2
*Oxy_2 5' *** 3' 59.2
*Bbp_Oxy_1 5' *** 3' 65.1
*Bbs_Oxy_2 5' *** 3' 66.9
(***)_S_for_1 5' *** 3' 71.2
(***)_S_rev_1 5' *** 3' 60.2
Bbp_(***)_S_for_1 5' *** 3' 70.9
Bbs_(***)_S_rev_1 5' *** 3' 63.3
(***)_H_up 5' *** 3' 69.9
(***)_H_low 5' *** 3' 70.6
Bbp_(***)_Up 5' *** 3' 63.3
Bbs_(***)_Low 5' *** 3' 57.7
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