From 2007.igem.org

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Revision as of 11:08, 3 August 2007

1. Mix :

-1 μg of plasmid

-3 μl of Buffer 10X

-0.3 μl BSA 100X

-3 units of restriction enzyme

-water up to 30 μl

2. Incubate at 37 °C for 1 hr.

3. Incubate at the inactivation temperature of the enzyme for 20 minutes.

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