Glasgow/Wetlab/Week6

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== Week 6 ==
== Week 6 ==
=== Monday 6th August 2007 ===
=== Monday 6th August 2007 ===
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Maia checked the presence and size of (*m*) and (*s*) inserts within the Topo vector by repeating the PCR performed on 02/08. Results confirmed presence of inserts with the correct size. The TOPO vectors containing (*m*) and (*s*) were then sent for sequencing to ensure the inserts did not contain any mistakes. DNA sequenced and the primers used are summarized in the table below.
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Gene Label Colonies Primers
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(*m*) B1 17, 18, 19, 26 (*m*)_for_1 + Methyl _2
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(*s*) B3 20 (*s*)_for_1  + Oxy_2
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(*m*) C5 25,24 (*m*)_for_1 + (*m*)_rev_1
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(NB - For each 2 x 10µl was sent)
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# Christine repeated the cloning of the 7-gene operon into TOPO vector using fresh PCR product. The PCR performed was a repeat of the PCR from 31/07. 4 µl of PCR product was cloned into TOPO vector (see protocol 12).
=== Tuesday 7th August 2007 ===
=== Tuesday 7th August 2007 ===

Revision as of 10:04, 16 August 2007

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Contents

Week 6

Monday 6th August 2007

  1. Maia checked the presence and size of (*m*) and (*s*) inserts within the Topo vector by repeating the PCR performed on 02/08. Results confirmed presence of inserts with the correct size. The TOPO vectors containing (*m*) and (*s*) were then sent for sequencing to ensure the inserts did not contain any mistakes. DNA sequenced and the primers used are summarized in the table below. Gene Label Colonies Primers (*m*) B1 17, 18, 19, 26 (*m*)_for_1 + Methyl _2 (*s*) B3 20 (*s*)_for_1 + Oxy_2 (*m*) C5 25,24 (*m*)_for_1 + (*m*)_rev_1 (NB - For each 2 x 10µl was sent)
    1. Christine repeated the cloning of the 7-gene operon into TOPO vector using fresh PCR product. The PCR performed was a repeat of the PCR from 31/07. 4 µl of PCR product was cloned into TOPO vector (see protocol 12).

    Tuesday 7th August 2007

    Wednesday 8th August 2007

    Thursday 9th August 2007

    === Friday 10th August 2007 ===
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