From 2007.igem.org

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Revision as of 11:31, 23 August 2007

Contents 1 5:50 Action points from last meeting: 2 Status Reports: (5 minutes each including any discussion) 3 New Agenda Items: 4 7:00 End 5 Igem Administrative Timetable: 6 Documentation wiki status:

5:50 Action points from last meeting:

• Alisa& Pat: Wiki "Parts_used status" up to date? -(outstanfding from 19/7/07)-Done
• Pat & Alisa: Plan for red light receptor on wiki -(Lac Z reporter outstanding )
• Alisa, Paul & Jan: Review blue light theory - done
• Jan:Theory for blue light on wiki-done
• Jan:ComP fusion to be ordered.- 31 August shipping
• Jan:Plan for blue light on wiki by 17th August- Done (needs more details- coundn't implement this)
• Jan:<b/>Order red and blue filters.-Phill to pick up
• <b>Paul: Status of #BBa-M30109 & #BBa-V1012 lost in transit?- Shipped
• Craig: Alkaline lysis technique for miniprep to be documented-link to open wetware?
• Cheng: Daisy to produce competent cells of DB3.1 and Enz- strains.- waiting on strains still
• Chris: Gas vesicle expression experiment to be run.

Status Reports: (5 minutes each including any discussion)

• Alisa: Ligation experiments progress.
  • Attempting ligation of RBS to LacZ reporter.
  • Phil to culture on Sunday
  • Phil & Pat to miniprep and digest on Monday
• Pat & Alisa: Red light receptor progress/design/plan- needs?
  • phase 1 osmolarity test of YFP under enz
  • phase 2 Red light driving YFP
  • Nothing planned for this week due to academic load.
• Jan: Blue light receptor design/plan - needs?
  • Nothing this week
• Phil: Gas vesicles progress/design/plan - needs?
  • Plan up to basic biobrick complete,
  • Second mutagenesis under way. Problem with first apears to be PCR fixed on second by increesing seg1 to 2 min, seg 2 to 1min and elongation to 15minutes
  • Chris to start expression experiment cultures
  • Phil to set up bouyancy test experiment with cultures on saturday and photograph throughout week
  • Craig to provide protocol for protein extraction
  • Need to check expression of gas vesicles Chris to perform.
  • Plan for support parts/test harness required.
  • Lac promotor and antibiotic death plasmids required.
• Kasia: Documentation progress vs last report/ new problems/ needs.-No report

New Agenda Items:

• Phil: Administrative Timetable: (10 Weeks to go)
• Next meeting: <b/> 21th August (Friday) 5.30PM.

7:00 End

Igem Administrative Timetable:

1 September	Final team rosters due.
1 September	Jamboree attendance fees due $100USD per team member.
26 October	Project and part documentation due DNA received USA.
26 October	BioBrick Part DNA received by the Registry.
3-4 November	iGEM Competition Jamboree, MIT, USA.

.

Documentation wiki status:

• • Priority should be on plans and experimental report analysis and conclusions-----Alisa .
  • If only Pat & Alisa doing lab work not much documentation needed.
  • Equipement:
    • MissingLas3000 method (in lab notebook)
    • Missing DNA measurement protocol (eppendorf)
  • Reagents pages incomplete:
    • Some location pictures not finished or uploaded.
    • Some newer reagents not entered.
  • Secondary reagents pages not made:
    • LB, Plates, TAE , NZY+ Broth
  • Protocol part:
    • No connections from Protocols to reagents.
    • Ligation protocol missing
  • Lab notebook:
    • Format uses too many lines to see connections between days.
    • Latter part missing links
    • Can table of contents be restricted to date level?
  • Experimental reports:
    • Lacks discussion/conclusion
    • Ligation experiment missing
    • Lattest few gells not marked up and entered.
  • Project overview link broken
  • Background:
    • GFP missing ,
    • Red photo sensor to GFP test harness missing.
  • Plan: Only Gas vesicles plan entered.

Individual	Desired time committment
Pat	About 4h/wk Monday afternoon or Tuesday morning
Alisa	About 4h/wk - Thursday afternoon
Jan	About 10h/pw - flexible weekday mornings
Phil	About 18h/pw - Thursday morning, Friday, Saturday or Sunday,