Week 2
From 2007.igem.org
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| - | *We | + | *We start plasmid amplification from IGEM 2007 plates. |
| - | *We | + | *We have some problems with Ampicillin solubility in water. We find that our Ampicillin was not soluble in water, so we get to try to resuspend it in NaOH 1M with a work concentration of 50 μg/ml. Since bacterial cells still grew on our plates, we try to change the antibiotic. |
| - | So, we | + | So, we use a water- soluble Ampicillin with a concentration of 100 μg/ml. We find that it finally worked. |
| - | *We also | + | *We also have some problems with Kanamycin. We first use it at a work concentration of 20 μg/ml, but it don’t work. Guys from the Boston University team have been so kind to tell us to use Kanamycin at a work concentration of 50 μg/ml and it workes correctly. |
*Preparation of [[Bologna_University/IPTG stocks preparation | IPTG stocks]] 100 mM | *Preparation of [[Bologna_University/IPTG stocks preparation | IPTG stocks]] 100 mM | ||
Revision as of 09:30, 27 August 2007
- 07/09/07 - 07/15/07
- We start plasmid amplification from IGEM 2007 plates.
- We have some problems with Ampicillin solubility in water. We find that our Ampicillin was not soluble in water, so we get to try to resuspend it in NaOH 1M with a work concentration of 50 μg/ml. Since bacterial cells still grew on our plates, we try to change the antibiotic.
So, we use a water- soluble Ampicillin with a concentration of 100 μg/ml. We find that it finally worked.
- We also have some problems with Kanamycin. We first use it at a work concentration of 20 μg/ml, but it don’t work. Guys from the Boston University team have been so kind to tell us to use Kanamycin at a work concentration of 50 μg/ml and it workes correctly.
- Preparation of IPTG stocks 100 mM
