Week 8
From 2007.igem.org
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::'''08/23/07''' | ::'''08/23/07''' | ||
| - | *A new R0051+P0412 (I763010) ligation a new protocol (3h 15°C and 1.2 ul | + | *A new R0051+P0412 (I763010) ligation with a new protocol (3h 15°C and 1.2 ul T4 Ligase). |
*R0051, I763012 digestion with Spe/Pst1. | *R0051, I763012 digestion with Spe/Pst1. | ||
*P0412, J04631 digestion with Xba/Pst1. | *P0412, J04631 digestion with Xba/Pst1. | ||
| Line 29: | Line 29: | ||
::'''08/24/07''' | ::'''08/24/07''' | ||
| - | *We | + | *We plan new amplifications. |
[[Bologna | Back]] | [[Bologna | Back]] | ||
Revision as of 13:57, 27 August 2007
- 08/20/07
- I763010 and C0012 trasformation.
- 08/21/07
- There isn't colonies for I763010 and C0012. we can't amplify this biobrick.
- J04631 digestion.
- Band extraction.
- R0051+P0412 (I763010)ligation.
- 08/22/07
- There isn't any colony for R0051+P0412 (I763010)
- 08/23/07
- A new R0051+P0412 (I763010) ligation with a new protocol (3h 15°C and 1.2 ul T4 Ligase).
- R0051, I763012 digestion with Spe/Pst1.
- P0412, J04631 digestion with Xba/Pst1.
- Run and extraction from gel.
- Transformation of R0051+P0412 (I763010) ligations.
- 08/24/07
- We plan new amplifications.
