Paris/August 27
From 2007.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| + | [[Paris/August 26|yesterday]] -- [[Paris/August 28|tomorrow]] | ||
| + | |||
==Transformation of DH5α strains == | ==Transformation of DH5α strains == | ||
Strain transformed with ATTB, ATTP and PUC19. | Strain transformed with ATTB, ATTP and PUC19. | ||
Revision as of 15:31, 27 August 2007
Transformation of DH5α strains
Strain transformed with ATTB, ATTP and PUC19.
1. Thaw TSS cells on ice.
2. Add 1μl DNA.
3. Let sit for 30 minutes on ice.
4. Incubate cells for 20 seconds at 42°C.
5. Incubate cells on ice for 2 min.
6. Add 1 mL LB at room temp.
7. Incubate for 1 hour at 37°C on shaker.
Error made at step 6 : LB with Kanamycine used.
8. Spread 100µL and 500µL onto different plates made with Kanamycine.
9. Grow overnight at 37 °C.
