From 2007.igem.org

Revision as of 06:56, 30 August 2007 (view source) Lihsiangyen (Talk | contribs) ← Older edit		Revision as of 06:57, 30 August 2007 (view source) Lihsiangyen (Talk | contribs) Newer edit →
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	**Emition: 527nm		**Emition: 527nm
	*Liquid culture for MGC clones		*Liquid culture for MGC clones
-	** INS(human), IGFBP3(human), IGFBP7(human), IDE(mouse)	+	** INS(human), IGFBP3(human), IGFBP7(human), IDE(mouse) for insulin project
-	** PUM1, AKAP1 for m.t. experiment	+	** PUM1, AKAP1 for m.t. project

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Revision as of 06:57, 30 August 2007

• Plasmid extraction:
  • pOmpC+EYFP 1-1, 1-2, 2, 3, 4, 5 (duplicate the first tube for even balance)
  • Extract from 1.5ml LB medium to 20ul EB buffer
  • Stored in -20°C
• EYFP spectral characteristics:
  • Excitation: 514nm
  • Emition: 527nm
• Liquid culture for MGC clones
  • INS(human), IGFBP3(human), IGFBP7(human), IDE(mouse) for insulin project
  • PUM1, AKAP1 for m.t. project