Paris/Oligos

From 2007.igem.org

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| CAGGAAACAGCTTATGAC
| CAGGAAACAGCTTATGAC
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| style="background: #ccffcc;" | 43
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| Bfr-FtsK-F
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| TTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAATGCCACCTGACGTCTAAGAA
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| 70
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|- style="background: #cccccc;"
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| style="background: #ccffcc;" | 44
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| Bfr-FtsK-R
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| CCAGGTTACCCCAGTACTGTTGCTGTATGGATTAACAGGAGTGTAATCAAATTACCGCCTTTGAGTGAGC
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| 70
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|- style="background: #cccccc;"
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| style="background: #ccffcc;" | 44
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| Aft-FtsK-F
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| AGGGGCACAACGGTAATCGTGAAGTGCTGGCCCCACCGCCGTTTGACTAATGCCACCTGACGTCTAAGAA
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|- style="background: #cccccc;"
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| style="background: #ccffcc;" | 44
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| Aft-FtsK-R
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| GAAAATACTGAATTTTCAGTTGATAGAGTGCCGGATGCGACGCTACCGCGATTACCGCCTTTGAGTGAGC
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| 70
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Revision as of 13:30, 28 September 2007

Table of Oligos
Number Name Sequence Length Comments
1 Lox71-FtsZ-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATT ATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT 89
2 FtsZ-R TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCAGGAAT 49
3 Lox71-FtsA-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACG AAGTTATTAATAATCAGGCACAGGCAGAACAACAATG 89
4 DEcoR1-FtsZ-R CGGTATTTACCGCGAAGAACTCAACACCTTCAATGCGCTC 40
5 DEcoR1-FtsZ-F TGCGCGAGCGCATTGAAGGTGTTGAGTTCTTCGCGGTAAA 40
6 Lox66-DapAColi-F TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACG AACGGTAGTTTGCACAGAGGATGGCCCATGTT 84
7 DapAColi-R TTTCTGCAGCGGCCGCTACTAGTATTATTACAGCAAACCGGCATGCTTAAGC 52
8 Lox66-DapASubtilis-F TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACG AACGGTAATCATGATGAGGTGAATAAAATGAA 84
9 DapASubtilis-R TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT 52
10 Lox71-KmR-F TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACG AAGTTATCTGATCCTTCAACTCAGCAAAAGTTC 85
11 Lox66-KmR-R TTTTACTAGTAGCGGCCGCTGCAGTACCGTTCGTATAATGTATGCTATACCA AGTTATTTATTAGAAAAACTCATCGAGCATCAAA 86
12 DPst1-DGAT-F AGTGCTGTCGTATATGGCCCAGCAGGACTCAACATAATTT 40
13 DPst1-DGAT-R AAATTATGTTGAGTCCTGCTGGGCCATATACGACAGCACT 40


14 Lox66-F AATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTATAC TAGTAGCGGCCGCTGCA
15 Lox66-R GCGGCCGCTACTAGTATACCGTTCGTATAATGTATGCTATACCAAGTTATCTCTAG AAGCGGCCGCG
16 Lox71-F AATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTACT AGTAGCGGCCGCTGCA
17 Lox71-R GCGGCCGCTACTAGTAATAACTTCGTATAATGTATCGTATACGAACGGTACTCTAG AAGCGGCCGCG
18 VF2 tgccacctgacgtctaagaa 20 Forward Primer to Biobrick vectors (VF2) BBa_G00100
19 VR attaccgcctttgagtgagc 20 Reverse Primer to Biobrick Vectors (VR) BBa_G00101
20 RBS-DapAColi-F TTTGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAATACTAGATGTT CACGGGAAGTATTGTCGCGA 71 Biobrick prefix + Strong RBS BBa_B0030 + 25 bp homology to DapA (Coli) from ATG
21 RBS-DapASubtilis-F TTTGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAATACTAGATGAA TTTCGGAAATGTGTCTACAG 71 Biobrick prefix + Strong RBS BBa_B0030 + 25 bp homology to DapA (Subtilis) from ATG
22 SEQFtsA-1F GTCGTCCATACCGCGAAATC 20
23 SEQFtsA-2F CGTGGAAGTGCCGAGCGTAG 20
24 SEQFtsZ-1F GGTATCGCTGAACTGATTAC 20
25 SEQDapASub-1F TTAAAGCAATTGCGGCAGAG 20
26 SEQDapAColi-1F CAATCGTCCGTCGCAAGAAG 20
27 ForDGAT1 ATCCACGCTATGCGCCCATTAC 22 Tm = 60°C
28 RevDGAT1 GGGCTAATTTAGCCCTTTAGTT 22 Tm = 50°C
29 ForDGAT_BB1 AAAGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAAATCCACGCTATGCGCCCATTAC 62 Tm = 60/83°C
30 RevDGAT_BB1 CTGCAGCGGCCGCTACTAGTAGGGCTAATTTAGCCCTTTAGTT 43 Tm = 50/74°C
31 DapAp-F aattcgcggccgcttctagagAACAATCAGAACGGTTCTGTCTGCTTGCTTTT AATGCCATACCAAACGTACCATTGAGACACTTGTTTGCACAGAGGATGGta 104
32 DapAp-R ctagtaCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCA TTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTctctagaagcggccgcg 104
33 dDapA-F CATACCAAACGTACCATTGAGACACTTGTTTGCACAGAGGAT GGCCCATGATTCCGGGGATCCGTCGACC 70 more here
34 dDapA-R AAGCCATCAAATCTCCCTAAACTTTACAGCAAACCGGCATGC TTAAGCGCTGTAGGCTGGAGCTGCTTCG 70 more here
35 dFtsZ-F GACGATGATTACGGCCTCAGGCGACAGGCACAAATCGGAGAGA AACTATGATTCCGGGGATCCGTCGACC 70 more here
36 dFtsZ-R GAAACCCAAATTCCAGTCAATTCTTAATCAGCTTGCTTACGCA GGAATGCTGTAGGCTGGAGCTGCTTCG 70 more here
37 dDapAverif-F ACAATCAGAACGGTTCTGTCTG 22
38 dDapAverif-R ATAGCGTGAGTCAGAACTACAG 22
39 dFtsZverif-R TCGCCAAATTACCTATCCAACC 22
40 SEQ-mRFP-R CGGACGACCTTCACCTTCAC
41 M13(-20)-F GTAAAACGACGGCCAG
42 M13-R CAGGAAACAGCTTATGAC
43 Bfr-FtsK-F TTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAATGCCACCTGACGTCTAAGAA 70
44 Bfr-FtsK-R CCAGGTTACCCCAGTACTGTTGCTGTATGGATTAACAGGAGTGTAATCAAATTACCGCCTTTGAGTGAGC 70
44 Aft-FtsK-F AGGGGCACAACGGTAATCGTGAAGTGCTGGCCCCACCGCCGTTTGACTAATGCCACCTGACGTCTAAGAA 70
44 Aft-FtsK-R GAAAATACTGAATTTTCAGTTGATAGAGTGCCGGATGCGACGCTACCGCGATTACCGCCTTTGAGTGAGC 70
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