Paris/Oligos
From 2007.igem.org
(Difference between revisions)
Nicolas C. (Talk | contribs) |
Nicolas C. (Talk | contribs) |
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| Line 17: | Line 17: | ||
| style="background: #ccffcc;" | O2 | | style="background: #ccffcc;" | O2 | ||
| FtsZ-R | | FtsZ-R | ||
| - | | | + | | TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCA GGAAT |
| 49 | | 49 | ||
| | | | ||
| Line 23: | Line 23: | ||
| style="background: #ccffcc;" | O3 | | style="background: #ccffcc;" | O3 | ||
| Lox71-FtsA-F | | Lox71-FtsA-F | ||
| - | | | + | | TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATT ATACGAAGTTATTAATAATCAGGCACAGGCAGAACAACAATG |
| 89 | | 89 | ||
| | | | ||
| Line 98: | Line 98: | ||
| style="background: #ccffcc;" | O14 | | style="background: #ccffcc;" | O14 | ||
| Lox66-F | | Lox66-F | ||
| - | | | + | | AATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAAC GGTATACTAGTAGCGGCCGCTGCA |
| | | | ||
| | | | ||
| Line 105: | Line 105: | ||
| style="background: #ccffcc;" | O15 | | style="background: #ccffcc;" | O15 | ||
| Lox66-R | | Lox66-R | ||
| - | | | + | | GCGGCCGCTACTAGTATACCGTTCGTATAATGTATGCTATACCAAGTTATC TCTAGAAGCGGCCGCG |
| | | | ||
| | | | ||
| Line 112: | Line 112: | ||
| style="background: #ccffcc;" | O16 | | style="background: #ccffcc;" | O16 | ||
| Lox71-F | | Lox71-F | ||
| - | | | + | | AATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAG TTATTACTAGTAGCGGCCGCTGCA |
| | | | ||
| | | | ||
| Line 119: | Line 119: | ||
| style="background: #ccffcc;" | O17 | | style="background: #ccffcc;" | O17 | ||
| Lox71-R | | Lox71-R | ||
| - | | | + | | GCGGCCGCTACTAGTAATAACTTCGTATAATGTATCGTATACGAACGGTAC TCTAGAAGCGGCCGCG |
| | | | ||
| | | | ||
| Line 140: | Line 140: | ||
| style="background: #ccffcc;" | O20 | | style="background: #ccffcc;" | O20 | ||
| RBS-DapAColi-F | | RBS-DapAColi-F | ||
| - | | | + | | TTTGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAATACTAGATGT TCACGGGAAGTATTGTCGCGA |
| 71 | | 71 | ||
| Biobrick prefix + Strong RBS BBa_B0030 + 25 bp homology to DapA (Coli) from ATG | | Biobrick prefix + Strong RBS BBa_B0030 + 25 bp homology to DapA (Coli) from ATG | ||
| Line 203: | Line 203: | ||
| style="background: #ccffcc;" | O29 | | style="background: #ccffcc;" | O29 | ||
| ForDGAT_BB1 | | ForDGAT_BB1 | ||
| - | | | + | | AAAGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAAATCCACGCTATG CGCCCATTAC |
| 62 | | 62 | ||
| Tm = 60/83°C | | Tm = 60/83°C | ||
| Line 289: | Line 289: | ||
| style="background: #ccffcc;" | O43 | | style="background: #ccffcc;" | O43 | ||
| Bfr-FtsK-F | | Bfr-FtsK-F | ||
| - | | | + | | TTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAAT GCCACCTGACGTCTAAGAA |
| 70 | | 70 | ||
| | | | ||
| Line 295: | Line 295: | ||
| style="background: #ccffcc;" | O44 | | style="background: #ccffcc;" | O44 | ||
| Bfr-FtsK-R | | Bfr-FtsK-R | ||
| - | | | + | | CCAGGTTACCCCAGTACTGTTGCTGTATGGATTAACAGGAGTGTAATCAAA TTACCGCCTTTGAGTGAGC |
| 70 | | 70 | ||
| | | | ||
| Line 301: | Line 301: | ||
| style="background: #ccffcc;" | O45 | | style="background: #ccffcc;" | O45 | ||
| Aft-FtsK-F | | Aft-FtsK-F | ||
| - | | | + | | AGGGGCACAACGGTAATCGTGAAGTGCTGGCCCCACCGCCGTTTGACTAAT GCCACCTGACGTCTAAGAA |
| 70 | | 70 | ||
| | | | ||
| Line 307: | Line 307: | ||
| style="background: #ccffcc;" | O46 | | style="background: #ccffcc;" | O46 | ||
| Aft-FtsK-R | | Aft-FtsK-R | ||
| - | | | + | | GAAAATACTGAATTTTCAGTTGATAGAGTGCCGGATGCGACGCTACCGCGA TTACCGCCTTTGAGTGAGC |
| 70 | | 70 | ||
| | | | ||
|} | |} | ||
Revision as of 20:47, 9 October 2007
| Table of Oligos | ||||
|---|---|---|---|---|
| Number | Name | Sequence | Length | Comments |
| O1 | Lox71-FtsZ-F | TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATT ATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT | 89 | |
| O2 | FtsZ-R | TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCA GGAAT | 49 | |
| O3 | Lox71-FtsA-F | TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATT ATACGAAGTTATTAATAATCAGGCACAGGCAGAACAACAATG | 89 | |
| O4 | DEcoR1-FtsZ-R | CGGTATTTACCGCGAAGAACTCAACACCTTCAATGCGCTC | 40 | |
| O5 | DEcoR1-FtsZ-F | TGCGCGAGCGCATTGAAGGTGTTGAGTTCTTCGCGGTAAA | 40 | |
| O6 | Lox66-DapAColi-F | TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACG AACGGTAGTTTGCACAGAGGATGGCCCATGTT | 84 | |
| O7 | DapAColi-R | TTTCTGCAGCGGCCGCTACTAGTATTATTACAGCAAACCGGCATGCTTAAGC | 52 | |
| O8 | Lox66-DapASubtilis-F | TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACG AACGGTAATCATGATGAGGTGAATAAAATGAA | 84 | |
| O9 | DapASubtilis-R | TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT | 52 | |
| O10 | Lox71-KmR-F | TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACG AAGTTATCTGATCCTTCAACTCAGCAAAAGTTC | 85 | |
| O11 | Lox66-KmR-R | TTTTACTAGTAGCGGCCGCTGCAGTACCGTTCGTATAATGTATGCTATACCA AGTTATTTATTAGAAAAACTCATCGAGCATCAAA | 86 | |
| O12 | DPst1-DGAT-F | AGTGCTGTCGTATATGGCCCAGCAGGACTCAACATAATTT | 40 | |
| O13 | DPst1-DGAT-R | AAATTATGTTGAGTCCTGCTGGGCCATATACGACAGCACT | 40 |
|
| O14 | Lox66-F | AATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAAC GGTATACTAGTAGCGGCCGCTGCA | ||
| O15 | Lox66-R | GCGGCCGCTACTAGTATACCGTTCGTATAATGTATGCTATACCAAGTTATC TCTAGAAGCGGCCGCG | ||
| O16 | Lox71-F | AATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAG TTATTACTAGTAGCGGCCGCTGCA | ||
| O17 | Lox71-R | GCGGCCGCTACTAGTAATAACTTCGTATAATGTATCGTATACGAACGGTAC TCTAGAAGCGGCCGCG | ||
| O18 | VF2 | tgccacctgacgtctaagaa | 20 | Forward Primer to Biobrick vectors (VF2) BBa_G00100 |
| O19 | VR | attaccgcctttgagtgagc | 20 | Reverse Primer to Biobrick Vectors (VR) BBa_G00101 |
| O20 | RBS-DapAColi-F | TTTGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAATACTAGATGT TCACGGGAAGTATTGTCGCGA | 71 | Biobrick prefix + Strong RBS BBa_B0030 + 25 bp homology to DapA (Coli) from ATG |
| O21 | RBS-DapASubtilis-F | TTTGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAATACTAGATGAA TTTCGGAAATGTGTCTACAG | 71 | Biobrick prefix + Strong RBS BBa_B0030 + 25 bp homology to DapA (Subtilis) from ATG |
| O22 | SEQFtsA-1F | GTCGTCCATACCGCGAAATC | 20 | |
| O23 | SEQFtsA-2F | CGTGGAAGTGCCGAGCGTAG | 20 | |
| O24 | SEQFtsZ-1F | GGTATCGCTGAACTGATTAC | 20 | |
| O25 | SEQDapASub-1F | TTAAAGCAATTGCGGCAGAG | 20 | |
| O26 | SEQDapAColi-1F | CAATCGTCCGTCGCAAGAAG | 20 | |
| O27 | ForDGAT1 | ATCCACGCTATGCGCCCATTAC | 22 | Tm = 60°C |
| O28 | RevDGAT1 | GGGCTAATTTAGCCCTTTAGTT | 22 | Tm = 50°C |
| O29 | ForDGAT_BB1 | AAAGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAAATCCACGCTATG CGCCCATTAC | 62 | Tm = 60/83°C |
| O30 | RevDGAT_BB1 | CTGCAGCGGCCGCTACTAGTAGGGCTAATTTAGCCCTTTAGTT | 43 | Tm = 50/74°C |
| O31 | DapAp-F | aattcgcggccgcttctagagAACAATCAGAACGGTTCTGTCTGCTTGCTTTT AATGCCATACCAAACGTACCATTGAGACACTTGTTTGCACAGAGGATGGta | 104 | |
| O32 | DapAp-R | ctagtaCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCA TTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTctctagaagcggccgcg | 104 | |
| O33 | dDapA-F | CATACCAAACGTACCATTGAGACACTTGTTTGCACAGAGGAT GGCCCATGATTCCGGGGATCCGTCGACC | 70 | more here |
| O34 | dDapA-R | AAGCCATCAAATCTCCCTAAACTTTACAGCAAACCGGCATGC TTAAGCGCTGTAGGCTGGAGCTGCTTCG | 70 | more here |
| O35 | dFtsZ-F | GACGATGATTACGGCCTCAGGCGACAGGCACAAATCGGAGAGA AACTATGATTCCGGGGATCCGTCGACC | 70 | more here |
| O36 | dFtsZ-R | GAAACCCAAATTCCAGTCAATTCTTAATCAGCTTGCTTACGCA GGAATGCTGTAGGCTGGAGCTGCTTCG | 70 | more here |
| O37 | dDapAverif-F | ACAATCAGAACGGTTCTGTCTG | 22 | |
| O38 | dDapAverif-R | ATAGCGTGAGTCAGAACTACAG | 22 | |
| O39 | dFtsZverif-R | TCGCCAAATTACCTATCCAACC | 22 | |
| O40 | SEQ-mRFP-R | CGGACGACCTTCACCTTCAC | ||
| O41 | M13(-20)-F | GTAAAACGACGGCCAG | ||
| O42 | M13-R | CAGGAAACAGCTTATGAC | ||
| O43 | Bfr-FtsK-F | TTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAAT GCCACCTGACGTCTAAGAA | 70 | |
| O44 | Bfr-FtsK-R | CCAGGTTACCCCAGTACTGTTGCTGTATGGATTAACAGGAGTGTAATCAAA TTACCGCCTTTGAGTGAGC | 70 | |
| O45 | Aft-FtsK-F | AGGGGCACAACGGTAATCGTGAAGTGCTGGCCCCACCGCCGTTTGACTAAT GCCACCTGACGTCTAAGAA | 70 | |
| O46 | Aft-FtsK-R | GAAAATACTGAATTTTCAGTTGATAGAGTGCCGGATGCGACGCTACCGCGA TTACCGCCTTTGAGTGAGC | 70 | |
