Samantha Liang Notebook
From 2007.igem.org
(Difference between revisions)
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'''For cassette between Cre and toxin: [rbs][ATG][Lox][Term][Lox]''' <br> | '''For cassette between Cre and toxin: [rbs][ATG][Lox][Term][Lox]''' <br> | ||
*Made [rbs][ATG] variants with different ribosome binding sites | *Made [rbs][ATG] variants with different ribosome binding sites | ||
| - | '' | + | ''I716201 is Bca1106A.Bca1112'' <br> |
| - | I716202 is Bca1106B.Bca1112 <br> | + | ''I716202 is Bca1106B.Bca1112'' <br> |
| - | + | '' I716203 is Bca1128A.Bca1112'' <br> | |
| - | I716204 is Bca1090.Bca1112 <br> | + | ''I716204 is Bca1090.Bca1112'' <br> |
*Made [Lox][Term] variants with 2 different terminators (will probably use Bca1092 version) | *Made [Lox][Term] variants with 2 different terminators (will probably use Bca1092 version) | ||
| - | '' I716205 is Bca1114.Bca1124 <br> | + | '' I716205 is Bca1114.Bca1124'' <br> |
| - | + | '' I716206 is Bca1114.Bca1092 ''<br> | |
*Tomorrow will make [Lox][Term][Lox] part | *Tomorrow will make [Lox][Term][Lox] part | ||
Revision as of 19:29, 4 June 2007
My Construction Files
My Sequencing Files
My Biobricks
--Samanthaliang 15:29, 4 June 2007 (EDT)
- Oligos to make biobricks of toxins are not yet in - maybe Wednesday or Thursday?
For cassette between Cre and toxin: [rbs][ATG][Lox][Term][Lox]
- Made [rbs][ATG] variants with different ribosome binding sites
I716201 is Bca1106A.Bca1112
I716202 is Bca1106B.Bca1112
I716203 is Bca1128A.Bca1112
I716204 is Bca1090.Bca1112
- Made [Lox][Term] variants with 2 different terminators (will probably use Bca1092 version)
I716205 is Bca1114.Bca1124
I716206 is Bca1114.Bca1092
- Tomorrow will make [Lox][Term][Lox] part
- Then will put the rbsATG together with LoxTermLox
eek so many construction files and ape files to make.
to do
