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Revision as of 13:09, 18 October 2007

<a href="http://www.krackeler.com/products/fid/2753">Unit Definition of Restriction Enzymes</a>

• One unit is defined as the amount of enzyme required to hydrolyze 1µg of λ DNA to completion in 60 minutes in a total reaction volume of 50 µl, under optimal assay conditions (salt concentration, pH, temperature).
• Some enzymes which do not, or rarely, cleave λ DNA. DNA are tested using another substrate; for example, enzymes such as Not I or Sfi I are tested with adenovirus DNA, and Fok I is tested using pBR322.

<a href="http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/setting_up_reaction.asp">Setting Up a Restriction Endonuclease Reaction</a>

<a href="http://www.promega.com/guides/re_guide/chaptwo/2_1.htm">Standard Restriction Enzyme Reactions</a>

<a href="http://www.neb.com/nebecomm/products/faqcategory1.asp">FAQs for Restriction Endonucleases</a>

<a href="http://www.ndsu.edu/instruct/mcclean/plsc731/dna/dna5.htm">Restriction Modification System</a>

<a href="http://www.everythingbio.com/glos/definition.php?word=star+activity">Definition of star activity</a>

• AatII
• AccI
• AciI
• AseI
• BamHI
• BanII
• BbsI
• BbvI
• BglII
• BsaBI
• BspEI
• EcoRI
• EcoRV
• HindIII
• MboI
• MspI
• NcoI
• NdeI
• NheI
• NotI
• PacI
• PstI
• RsaI
• SacI
• SalI
• SmaI
• SpeI
• SwaI
• XbaI
• XhoI
• XmaI
• XmnI

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