BerkiGEM2007-SamanthaConstructionFile9
From 2007.igem.org
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NOT DONE YET...found an ALWNI site in Cre, use BglI instead | NOT DONE YET...found an ALWNI site in Cre, use BglI instead | ||
| - | '''[rbs][ | + | '''[rbs][Cre]''' <br> |
| - | ''' | + | '''rbs variants Bca1090''' <br> |
'''2 Different Cre variants (212 and 213)''' <br> | '''2 Different Cre variants (212 and 213)''' <br> | ||
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| - | + | Digest pBca9145-Bca1090 (BamHI/BglI/AlwNI, 942+596+553bp, Large) <br> | |
| - | Digest pBca9145- | + | |
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Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small) <br> | Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small) <br> | ||
Product is pBca9145-I716217. <br> | Product is pBca9145-I716217. <br> | ||
| - | + | Digest pBca9145-Bca1090 (BamHI/BglI, 942+596+553bp, Large) <br> | |
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| - | Digest pBca9145-Bca1090 (BamHI/BglI, | + | |
Digest pBca9145-I716213 (BglII/BglI, 2187+923bp, Small)<br> | Digest pBca9145-I716213 (BglII/BglI, 2187+923bp, Small)<br> | ||
Product is pBca9145-I716221. <br> | Product is pBca9145-I716221. <br> | ||
Revision as of 00:20, 8 June 2007
NOT DONE YET...found an ALWNI site in Cre, use BglI instead
[rbs][Cre]
rbs variants Bca1090
2 Different Cre variants (212 and 213)
Digest pBca9145-Bca1090 (BamHI/BglI/AlwNI, 942+596+553bp, Large)
Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small)
Product is pBca9145-I716217.
Digest pBca9145-Bca1090 (BamHI/BglI, 942+596+553bp, Large)
Digest pBca9145-I716213 (BglII/BglI, 2187+923bp, Small)
Product is pBca9145-I716221.
