Paris/October 19
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| + | | style="background: #ccffcc;" |D22bis | ||
| + | |pSB1A2 BBv | ||
| + | |MP6 | ||
| + | |EcoRI | ||
| + | |PstI | ||
| + | | | ||
| + | |pSB1A2 vector digested with EcoRI and PstI enzymes | ||
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|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D76 | | style="background: #ccffcc;" |D76 | ||
Revision as of 13:48, 19 October 2007
Digestion reactions
| Digestion Products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Size | Description | |||
| D22bis | pSB1A2 BBv | MP6 | EcoRI | PstI | pSB1A2 vector digested with EcoRI and PstI enzymes | ||||
| D76 | DGAT BB | P14(19/10/07) | EcoRI | PstI | DGAT (delta PstI) | ||||
| D79 | DGAT BI | P14(19/10/07) | XbaI | PstI | DGAT(delta PstI)PCR product for cloming as a BB in D47 | ||||
| D80 | PacI[ptet>>lox71-gfpT-lox66-mrfp]SmaI | P15 | PacI | SmaI | ptet>>lox71-gfpT-lox66-mrfp P15 PCR product digested by PacI-SmaI for cloning into pY-2C-IntC | ||||
| D81 | PacI[pY-2C-IntC]SmaI | pY-2C-IntC plasmid | PacI | SmaI | pY-2C-IntC digested for ptet>>lox71-gfpT-lox66-mrfp cloning | ||||
| D82 | frt1-CmR-frt2 FI | P16 | EcoRI | SpeI | frt1-CmR-frt2 PCR product digested for use as a FI | ||||
| D83 | frt1-CmR-frt2 BI | P16 | XbaI | PstI | frt1-CmR-frt2 PCR product digested for use as a BI | ||||
| D84 | frt1-CmR-frt2 BBi | P16 | EcoRI | PstI | frt1-CmR-frt2 PCR product digested for cloning as a BB | ||||
| D85 | frt1-CmR-frt2-T1T2 FI | P17 | EcoRI | SpeI | frt1-CmR-frt2-T1T2 PCR product digested for use as a FI | ||||
| D86 | frt1-CmR-frt2-T1T2 BI | P17 | XbaI- | PstI | frt1-CmR-frt2-T1T2 PCR product digested for use as a BI | ||||
| D87 | frt1-CmR-frt2-T1T2 BBi | P17 | EcoRI | PstI | frt1-CmR-frt2-T1T2 PCR product digested for cloning as a BB | ||||
| D88 | pTet>>lox71-gfptripqrt FV | L34 | EcoRI | XbaI | pTet>>lox71-gfptripqrt pSB1A2 dig. for use as a FV | ||||
| D89 | T-lox66-rbsdapAcoli BV | L53 | SpeI | PstI | T-lox66-rbsdapAcoli pSB1A2 dig. for use as a BV | ||||
| D90 | T-lox66-rbsdapAsubtilis BV | L56 | SpeI | PstI | T-lox66-rbsdapAsubtilis pSB1A2 dig. for use as a BV | ||||
| D91 | ptet>>lox71-gfpT-lox66-mrfp pSB1A2 BV | L59 | SpeI | PstI | ptet>>lox71-gfpT-lox66-mrfp pSB1A2 dig. for use as a BV | ||||
| D91 | rbsdapAcoli BI | L39 | XbaI | PstI | rbsdapAcoli extracted for use as a BI | ||||
| D92 | rbsdapAsubtilis BI | L64 | XbaI | PstI | rbsdapAsubtilis extracted for use as a BI | ||||
