Tokyo/Experimental precision and accurecy
From 2007.igem.org
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== AHL assay== | == AHL assay== | ||
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===Purpose1: === | ===Purpose1: === | ||
To see how precisely we determine the fluorescence from GFP by removing LB media. | To see how precisely we determine the fluorescence from GFP by removing LB media. | ||
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===Procedure: === | ===Procedure: === | ||
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| - | + | [[Tokyo/AHL assay Standard protocol |AHL assay Standard protocol ]] | |
| + | <br>Make 200 ul of mixtures of pos. con. and neg. con. | ||
| + | <br>The mixture ratios of pos. con. and neg. con. are | ||
| + | <br>(100:0), (70:30), (50:50), (30:70), (10:90), (3:97), (1:99) | ||
| + | <br>[[Tokyo/Wash |Wash]] | ||
===Result: === | ===Result: === | ||
===Conclusion: === | ===Conclusion: === | ||
Revision as of 17:01, 20 October 2007
Contents |
AHL assay
Purpose1:
To see how precisely we determine the fluorescence from GFP by removing LB media.
Samples:
placQI + GFP in pTrc99A (pos. con.)
A4 ΔP+ GFP (neg. con.)
Procedure:
AHL assay Standard protocol
Make 200 ul of mixtures of pos. con. and neg. con.
The mixture ratios of pos. con. and neg. con. are
(100:0), (70:30), (50:50), (30:70), (10:90), (3:97), (1:99)
Wash
